Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets
文献类型：期刊论文
| 作者 | Zhao, Xian-En; Zuo, Ya-Nan; Qu, Xiaoqing; Sun, Jing ; Liu, Lingyuan; Zhu, Shuyun
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| 刊名 | MICROCHIMICA ACTA
 |
| 出版日期 | 2019 |
| 卷号 | 186期号:12 |
| 关键词 | Two-dimensional nanomaterials Oxidase mimic Nanozyme Dopamine Hydrogen peroxide 3,3 ',5,5 '-Tetramethylbenzidine Catalytic oxidation Enzyme activities Human serum analysis |
| 英文摘要 | The authors describe novel colorimetric assays for tyrosinase (TYR) and catalase (CAT) based on the substrate-triggered decomposition of MnO2 nanosheets (NSs). The MnO2 NSs can act as oxidase mimics that catalyze the oxidation of the substrate tetramethylbenzidine (TMB) to form a blue dye with an absorption maximum at 652 nm. The oxidase-mimicking activity of the MnO2 NSs is inhibited by dopamine (DA)/hydrogen peroxide (H2O2) due to their decomposition of the MnO2 NSs. TYR catalyzes the oxidation of DA while CAT can decompose H2O2 into water and oxygen. Therefore, the oxidase-mimicking activity of MnO2 NSs is restored in the presence of both enzymes and their substrates. Based on the competitive consumption of substrates between enzymes and MnO2 NSs, a colorimetric method for determination of enzyme activity and its substrate is developed. The detection limits for TYR and CAT are 6 mU center dot mL(-1) and 33 mU center dot mL(-1), respectively. |
| 源URL | [http://210.75.249.4/handle/363003/60488]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, Xian-En,Zuo, Ya-Nan,Qu, Xiaoqing,et al. Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets[J]. MICROCHIMICA ACTA,2019,186(12). |
| APA | Zhao, Xian-En,Zuo, Ya-Nan,Qu, Xiaoqing,Sun, Jing,Liu, Lingyuan,&Zhu, Shuyun.(2019).Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets.MICROCHIMICA ACTA,186(12). |
| MLA | Zhao, Xian-En,et al."Colorimetric determination of the activities of tyrosinase and catalase via substrate-triggered decomposition of MnO2 nanosheets".MICROCHIMICA ACTA 186.12(2019). |
入库方式： OAI收割
来源：西北高原生物研究所
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