水稻OsWRKY78与GFP融合基因的拟南芥转化及亚细胞定位(英文)
文献类型：期刊论文
| 作者 | 刘顺枝1; 张美2; 唐馨3; 王小兰1 |
| 刊名 | Agricultural Science & Technology
 |
| 出版日期 | 2012 |
| 卷号 | 13期号:07页码:1395-1398 |
| 关键词 | words OsWRKY78 gene Green fluorescent protein gene Expression vector Subcellular localization |
| 英文摘要 | [Objective] The study was to understand the subcellular localization of OsWRKY78 protein in plants. [Method] Primers specific for OsWRKY78 gene were designed according to the OsWRKY78 full length sequence in Genbank. The gene was cloned by RT-PCR method. The gene was then recombined into a plasmid ex-pression vector carrying green fluorescent protein (GFP) gene, pBinGFP. The re- combinant was confirmed by PCR and enzyme digestion. The recombinant plasmid pBinGFP-OsWRKY was transformed into Arabidopsis through Agrobacterium tume-faciens strain GV3101 and transgenic plants were obtained. [Result] Measured by fluorescence microscopy, the expression of OsWRKY78 and GFP fusion protein in root tip cells was localized in the nucleus. [Conclusion] This study laid the foundation for further investigating the function of OsWRKY78 gene and its role in related sig- nal transduction and provided theoretical basis for exploring the relation between OsWRKY78 gene and brown planthoppers. |
| 源URL | [http://210.77.82.179/handle/2SCSIERX/2975]  |
| 专题 | 研究领域 |
| 作者单位 | 1.School of Biological Sciences,Guangzhou University, Guangzhou 510006, China; 2.South China Botanical Garden, Chinese Academy of Sciences, Guangdong 510650, China; 3.School of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China |
| 推荐引用方式 GB/T 7714 | 刘顺枝,张美,唐馨,等. 水稻OsWRKY78与GFP融合基因的拟南芥转化及亚细胞定位(英文)[J]. Agricultural Science & Technology,2012,13(07):1395-1398. |
| APA | 刘顺枝,张美,唐馨,&王小兰.(2012).水稻OsWRKY78与GFP融合基因的拟南芥转化及亚细胞定位(英文).Agricultural Science & Technology,13(07),1395-1398. |
| MLA | 刘顺枝,et al."水稻OsWRKY78与GFP融合基因的拟南芥转化及亚细胞定位(英文)".Agricultural Science & Technology 13.07(2012):1395-1398. |
入库方式： OAI收割
来源：华南植物园
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