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Comprehensive evaluation of the antioxidant capacity ofSceptridium ternatumusing multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis

文献类型：期刊论文

作者Zhu, Junfeng1,2; Zhong, Like1,2; Kong, Sisi1,2; Zhang, Yuting3; Huang, Ping1,2
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2020-08-09
关键词antioxidants colorimetric methods herbal medicine mass spectrometry
ISSN号1615-9306
DOI10.1002/jssc.202000550
通讯作者Zhu, Junfeng(zhujf@zjcc.org.cn) ; Huang, Ping(huang-ping1841@zjcc.org.cn)
英文摘要Sceptridium ternatumis a medicinal herb with multiple health benefits. However, its antioxidant activity and active components have not been clarified. In this study, the antioxidant capacity ofS. ternatumwas comprehensively investigated using multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis. First, the phenolic content, flavonoid content, and radical scavenging ability ofS. ternatumwere parallelly determined using colorimetric methods performed in 96-well microplates. The flavonoid content, rather than the phenolic content, was highly correlated with its antioxidant activity.Sceptridium ternatumwas shown to be a rich source of flavonoids, with a highest flavonoid yield of 3.44 +/- 0.11 mg/g. Subsequently, 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography experiment and quadrupole time-of-flight mass spectrometry analyses were carried out for rapid screening of the individual antioxidants. A total of 14O-glycosyl flavonoids with quercetin or kaempferol aglycone have been characterized. Particularly, quercetin 3-O-rhamnoside-7-O-glucoside exhibited the most potent antioxidant ability. Its half-maximal effective concentrations for scavenging 1,1-diphenyl-2-picrylhydrazyl and 2,2MODIFIER LETTER PRIME-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radicals were 70.55 +/- 2.69 and 106.90 +/- 1.76 mu g/mL, respectively, which were comparable with those ofl-ascorbic acid. Our results indicated that the combined colorimetric and chromatographic methods provided a practical strategy for the discovery of bioactive compounds from natural products.
WOS关键词Q-TOF-MS/MS ; OXIDATIVE STRESS ; SCEPTRIDIUM-TERNATUM ; FLAVONOID GLYCOSIDES ; BOTRYCHIUM ; IDENTIFICATION ; INFLAMMATION ; COMPONENTS ; EXTRACTS ; CLEAVAGE
资助项目Zhejiang Provincial Natural Science Foundation of China[LQ19H280002] ; Zhejiang Provincial Natural Science Foundation of China[YY18H310023] ; Hospital Pharmacy Research Project from Zhejiang Pharmaceutical Association[2018ZYY08] ; Projects of Medical and Health Technology Program in Zhejiang Province[2018KY286] ; Projects of Medical and Health Technology Program in Zhejiang Province[2017KY244] ; Projects of Medical and Health Technology Program in Zhejiang Province[2016KYB033] ; Projects of Medical and Health Technology Program in Zhejiang Province[2015KYB061] ; 30 Talent Program of Cancer Hospital of the University of Chinese Academy of Sciences
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000557373300001
出版者WILEY-V C H VERLAG GMBH
资助机构Zhejiang Provincial Natural Science Foundation of China ; Hospital Pharmacy Research Project from Zhejiang Pharmaceutical Association ; Projects of Medical and Health Technology Program in Zhejiang Province ; 30 Talent Program of Cancer Hospital of the University of Chinese Academy of Sciences
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/45038]  
专题中国科学院合肥物质科学研究院
通讯作者Zhu, Junfeng; Huang, Ping
作者单位1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Lab Clin Pharm, Hangzhou, Peoples R China
2.Chinese Acad Sci, Inst Canc & Basic Med ICBM, Hangzhou 310022, Peoples R China
3.Hangzhou Med Coll, Hangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Zhu, Junfeng,Zhong, Like,Kong, Sisi,et al. Comprehensive evaluation of the antioxidant capacity ofSceptridium ternatumusing multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis[J]. JOURNAL OF SEPARATION SCIENCE,2020.
APA Zhu, Junfeng,Zhong, Like,Kong, Sisi,Zhang, Yuting,&Huang, Ping.(2020).Comprehensive evaluation of the antioxidant capacity ofSceptridium ternatumusing multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis.JOURNAL OF SEPARATION SCIENCE.
MLA Zhu, Junfeng,et al."Comprehensive evaluation of the antioxidant capacity ofSceptridium ternatumusing multiple colorimetric methods and 1,1-diphenyl-2-picrylhydrazyl-high-performance liquid chromatography analysis".JOURNAL OF SEPARATION SCIENCE (2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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