A high throughput plant regeneration system from shoot stems of Sapium sebiferum Roxb., a potential multipurpose bioenergy tree
文献类型：期刊论文
| 作者 | Hou, Jinyan1 ; Mao, Yingji2; Su, Pengfei1,3; Wang, Dacheng1,3; Chen, Xue1; Huang, Shengwei1; Ni, Jun1; Zhao, Weiwei1; Wu, Lifang1,3,4
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| 刊名 | INDUSTRIAL CROPS AND PRODUCTS
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| 出版日期 | 2020-10-15 |
| 卷号 | 154 |
| ISSN号 | 0926-6690 |
| 关键词 | Chinese tallow tree Explant orientation Adventitious shoot Culture vessel type Ex vitro rooting ISSR |
| DOI | 10.1016/j.indcrop.2020.112653 |
| 通讯作者 | Wu, Lifang(lfwu@ipp.ac.cn) |
| 英文摘要 | Sapium sebiferum Roxb. is an economically important multipurpose woody plant in terms of its biodiesel-producing, medicinal and ornamental values. In the present study, a high throughput in vitro regeneration system of S. sebiferum through direct shoot organogenesis from shoot stems was developed. The effects of plant growth regulators (PGRs), explant orientation, culture vessel type, explant type and explant source on adventitious shoot induction were investigated. Adventitious shoots were induced from cut ends of shoot stems with or without axillary buds when they were cultivated on the Murashige and Skoog (MS) medium fortified with 6-benzylaminopurine (6-BA). The highest rate of shoot induction (100 %) with mean number of shoot buds (21.4 +/- 0.2) per explant was obtained when in vitro shoot stems without axillary buds were placed horizontally on the medium supplemented with 0.5 mg l(-1) 6-BA in glass petri dish. The induced shoot buds were transferred to MS medium supplemented with 0.5 mg l(-1) 6-BA and 0.1 mg l(-1) gibberellic acid (GA(3)) for shoot proliferation and elongation. The elongated shoots were continuously propagated through shoot stem sectioning until used for roofing. For in vitro adventitious root induction, the elongated shoots were cultivated on the MS basal medium supplemented with different strengths of macronutrient, various concentrations of auxins and sucrose. The highest root induction (100 %) was achieved when the elongated shoots were cultured on 1/8 MS medium fortified with 2.0 % sucrose and 0.5 mg l(-1) indole-3-butyric acid (IBA). For ex vitro roofing, 98.3 % shoots rooted when they were pretreated with IBA (1000 mg l(-1)) for 60 s. The plantlets rooted by both methods were successfully acclimatized and showed normal development with 91.4 % and 98.1 % survival rate, respectively. The genetic fidelity of the regenerants was confirmed by inter simple sequence repeat (ISSR) markers. The present in vitro regeneration protocol will play an important role in large-scale propagation, germplasm conservation as well as genetic improvement of this economically important multipurpose plant species. |
| WOS关键词 | CHINESE TALLOW TREE ; IN-VITRO REGENERATION ; JATROPHA-CURCAS L. ; CLONAL PROPAGATION ; GENETIC STABILITY ; GROWTH-REGULATORS ; AUXIN ; ANTIOXIDANT ; BIODIESEL ; FIDELITY |
| 资助项目 | Natural Science Foundation of Anhui province[1808085QC73] ; Supplementary Project of Science and Technology Service program of Chinese Academy of Sciences in Fujian province[2019T3031] ; National Key R&D Program of China[2018YFD0502001] ; National Key R&D Program of China[2018YFD0300901] |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000554526900040 |
| 资助机构 | Natural Science Foundation of Anhui province ; Supplementary Project of Science and Technology Service program of Chinese Academy of Sciences in Fujian province ; National Key R&D Program of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/45065]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wu, Lifang |
| 作者单位 | 1.Chinese Acad Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China 2.Bengbu Med Coll, Sch Life Sci, 2600 Donghai Rd, Bengbu 233030, Anhui, Peoples R China 3.Anhui Univ, Sch Life Sci, Hefei 230039, Anhui, Peoples R China 4.Chinese Acad Sci, Taihe Expt Stn, Hefei Inst Phys Sci, Taihe 236626, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hou, Jinyan,Mao, Yingji,Su, Pengfei,et al. A high throughput plant regeneration system from shoot stems of Sapium sebiferum Roxb., a potential multipurpose bioenergy tree[J]. INDUSTRIAL CROPS AND PRODUCTS,2020,154. |
| APA | Hou, Jinyan.,Mao, Yingji.,Su, Pengfei.,Wang, Dacheng.,Chen, Xue.,...&Wu, Lifang.(2020).A high throughput plant regeneration system from shoot stems of Sapium sebiferum Roxb., a potential multipurpose bioenergy tree.INDUSTRIAL CROPS AND PRODUCTS,154. |
| MLA | Hou, Jinyan,et al."A high throughput plant regeneration system from shoot stems of Sapium sebiferum Roxb., a potential multipurpose bioenergy tree".INDUSTRIAL CROPS AND PRODUCTS 154(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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