Accumulation of 2-Keto-L-Gulonate at 33℃ by a Thermotolerant Gluconobacter Oxydans Mutant Obtained by Ion Beam Implantation
文献类型：期刊论文
| 作者 | Yan Bing1; Xu An1 ; Zhang Wan1; Zhou Wei1; Zhang Jun1; Yao Jianming1; Yu Zengliang1
|
| 刊名 | Plasma Science & Technology
 |
| 出版日期 | 2006 |
| 卷号 | 8 |
| 关键词 | L-sorbose to 2-keto-L-gulonate transformation rate thermotolerant mutants ion beam |
| ISSN号 | 1009-0630 |
| 其他题名 | Accumulation of 2-Keto-L-Gulonate at 33℃ by a Thermotolerant Gluconobacter Oxydans Mutant Obtained by Ion Beam Implantation |
| 英文摘要 | To obtain thermotolerant mutants of G. oxydans, which can enhance the transformation rate of L-sorbose to 2-Keto-L-gulonate （2-KLG） at 33℃ in a two-step process of vitamin C manufacture, ion beam was used as a mutation source. Gluconobacter oxydans GO and Bacillus megaterium B0 were used in this study. The original strain Gluconobacter oxydans GO was mutated by the heavy ion implantation facility at the Institute of Plasma Physics, Chinese Academy of Sciences. Several mutants including Gluconobacter oxydans GI13 were isolated and cocultured with Bacillus megaterium B0 at 33℃ in shaking flasks. The average transformation rate of the new mixed strain GI13-B0 in per gram-molecule reached 94.4% after seven passages in shaking flasks, which was increased by 7% when compared with the original mixed strain G0-B0 （Gluconobacter oxydans GO and Bacillus megaterium B0）. Moreover, the transformation rate of I13B0 was stable at 94% at temperatures ranging from 25℃ to 33℃, which would be of much value in reducing energy consumption in the manufacture of L-ascorbic acid, especially in the season of summer. To clarify some mechanism of the mutation, the specific activities of L-sorbose dehydrogenase in both GO and GI13 were estimated. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:2631558 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/57070]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 作者单位 | 1.Institute of Plasma Physics, Chinese Academy of Sciences 2.Institute of Plasma Physics, Chinese Academy of Sciences 3.Institute of Plasma Physics, Chinese Academy of Sciences 4.Institute of Plasma Physics, Chinese Academy of Sciences 5.Institute of Plasma Physics, Chinese Academy of Sciences 6.Institute of Plasma Physics, Chinese Academy of Sciences 7.Institute of Plasma Physics, Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Yan Bing,Xu An,Zhang Wan,et al. Accumulation of 2-Keto-L-Gulonate at 33℃ by a Thermotolerant Gluconobacter Oxydans Mutant Obtained by Ion Beam Implantation[J]. Plasma Science & Technology,2006,8. |
| APA | Yan Bing.,Xu An.,Zhang Wan.,Zhou Wei.,Zhang Jun.,...&Yu Zengliang.(2006).Accumulation of 2-Keto-L-Gulonate at 33℃ by a Thermotolerant Gluconobacter Oxydans Mutant Obtained by Ion Beam Implantation.Plasma Science & Technology,8. |
| MLA | Yan Bing,et al."Accumulation of 2-Keto-L-Gulonate at 33℃ by a Thermotolerant Gluconobacter Oxydans Mutant Obtained by Ion Beam Implantation".Plasma Science & Technology 8(2006). |
入库方式： OAI收割
来源：合肥物质科学研究院
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