Antitumor and radiosensitization effect of ~(12)C~(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy
文献类型：期刊论文
| 作者 | Liu Hui1; Jin Chufeng1 ; Ge Shengfang2; Wu Lijun3
|
| 刊名 | Nuclear Science and Techniques
 |
| 出版日期 | 2016 |
| 卷号 | 27 |
| 关键词 | Radio genetic therapy ~(12)C ion irradiation Apoptosis Egr-1 prompter Tumor necrosis factor-related apoptosis-inducing ligand |
| ISSN号 | 1001-8042 |
| 其他题名 | Antitumor and radiosensitization effect of ~(12)C~(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy |
| 英文摘要 | Radio genetic therapy which combines gene therapy with radiotherapy has shown promising results in cancer treatment. In this study, an oncolytic adenovirusbased gene therapy system regulated by radiation was constructed to improve the cancer curative effect. This gene therapy system incorporated the radiation-inducible early growth response gene (Egr-1) promoter and the anticancer gene tumor necrosis factor-related apoptosis-inducing ligand (TRAIL). To confirm the antitumor effect of Ad-ET combined with ~(12)C~(6+) ion irradiation, the survival and apoptosis fraction of tumor cells HT1080 and normal cells MRC-5 in combination treatment were detected by CCK-8 assay and FACS analysis. Then the expression levels of TRAIL gene and protein were tested by real-time PCR and western blotting. The results show that ~(12)C~(6+) ion irradiation could induce cell growth inhibition and apoptosis by activating the TRAIL gene expression in tumor cells, while exhibiting no obvious toxicity to the normal lung cell line MRC-5. The results also demonstrate that use of an oncolytic adenovirusbased radiation-inducible gene therapy system together with ~(12)C~(6+) ion irradiation could cause synergistic antitumor effect specifically in tumor cells but not in normal cells. The results indicate that the novel radio genetic therapy could potentiate radiation treatment by improving the safety and efficiency of monotherapy, and provide theoretical support for clinical application of combination treatment. |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5661469 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/58159]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 作者单位 | 1.Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences 2.Institute of Nuclear Energy Safety Technology, Chinese Academy of Sciences 3.Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University 4.Institute of Technical Biology and Agriculture Engineering, Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Liu Hui,Jin Chufeng,Ge Shengfang,et al. Antitumor and radiosensitization effect of ~(12)C~(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy[J]. Nuclear Science and Techniques,2016,27. |
| APA | Liu Hui,Jin Chufeng,Ge Shengfang,&Wu Lijun.(2016).Antitumor and radiosensitization effect of ~(12)C~(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy.Nuclear Science and Techniques,27. |
| MLA | Liu Hui,et al."Antitumor and radiosensitization effect of ~(12)C~(6+) heavy-ion irradiation mediated by radiation-inducible gene therapy".Nuclear Science and Techniques 27(2016). |
入库方式： OAI收割
来源：合肥物质科学研究院
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