Aptamer-based optical manipulation of protein subcellular localization in cells
文献类型：期刊论文
| 作者 | Xie, Sitao1; Du, Yulin1; Zhang, Yu1; Wang, Zhimin1; Zhang, Dailiang1; He, Lei1; Qiu, Liping1; Jiang, Jianhui1; Tan, Weihong1,2,3,4 |
| 刊名 | NATURE COMMUNICATIONS
 |
| 出版日期 | 2020-03-12 |
| 卷号 | 11 |
| ISSN号 | 2041-1723 |
| DOI | 10.1038/s41467-020-15113-2 |
| 通讯作者 | Qiu, Liping(qiuliping@hnu.edu.cn) ; Jiang, Jianhui(jianhuijiang@hnu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn) |
| 英文摘要 | Protein-dominant cellular processes cannot be fully decoded without precise manipulation of their activity and localization in living cells. Advances in optogenetics have allowed spatio-temporal control over cellular proteins with molecular specificity; however, these methods require recombinant expression of fusion proteins, possibly leading to conflicting results. Instead of modifying proteins of interest, in this work, we focus on design of a tunable recognition unit and develop an aptamer-based near-infrared (NIR) light-responsive nano-platform for manipulating the subcellular localization of specific proteins in their native states. Our results demonstrate that this nanoplatform allows photocontrol over the cytoplasmicnuclear shuttling behavior of the target RelA protein (a member of the NF-kappa beta family), enabling regulation of RelA-related signaling pathways. With a modular design, this aptamer-based nanoplatform can be readily extended for the manipulation of different proteins (e.g., lyso-zyme and p53), holding great potential to develop a variety of label-free protein photoregulation strategies for studying complex biological events. |
| WOS关键词 | OPTOGENETIC CONTROL ; UP-CONVERSION ; DNA ; SELECTION ; ALPHA ; SPACE |
| 资助项目 | NSFC[NSFC 21827811] ; NSFC[21527810] ; NSFC[21922404] ; National Key Research Program[2019YFA0905800] ; Science and Technology Project of Hunan Province[2017XK2103] ; Hunan Provincial Innovation Foundation For Postgraduate |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000563527600008 |
| 出版者 | NATURE PUBLISHING GROUP |
| 资助机构 | NSFC ; National Key Research Program ; Science and Technology Project of Hunan Province ; Hunan Provincial Innovation Foundation For Postgraduate |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/70498]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Qiu, Liping; Jiang, Jianhui; Tan, Weihong |
| 作者单位 | 1.Hunan Univ, Collaborat Innovat Ctr Chem & Mol Med, Coll Chem & Chem Engn,Mol Sci & Biomed Lab MBL, Coll Biol,State Key Lab Chemo Biosensing & Chemom, Changsha 410082, Peoples R China 2.Chinese Acad Sci, Canc Hosp, Univ Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 3.Shanghai Jiao Tong Univ, Renji Hosp, State Key Lab Oncogenes & Related Genes, Sch Med,Inst Mol Med IMM, Shanghai 200240, Peoples R China 4.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Sitao,Du, Yulin,Zhang, Yu,et al. Aptamer-based optical manipulation of protein subcellular localization in cells[J]. NATURE COMMUNICATIONS,2020,11. |
| APA | Xie, Sitao.,Du, Yulin.,Zhang, Yu.,Wang, Zhimin.,Zhang, Dailiang.,...&Tan, Weihong.(2020).Aptamer-based optical manipulation of protein subcellular localization in cells.NATURE COMMUNICATIONS,11. |
| MLA | Xie, Sitao,et al."Aptamer-based optical manipulation of protein subcellular localization in cells".NATURE COMMUNICATIONS 11(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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