A highly sensitive DNAzyme-based SERS biosensor for quantitative detection of lead ions in human serum
文献类型：期刊论文
| 作者 | Xu, Wei1,2,3 ; Zhao, Aiwu1,2,3; Zu, Fangtao1,2,3; Khan, Ranjha4; Hussain, Hafiz Muhammad Jafar4; Li, Jian5
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| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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| 出版日期 | 2020-07-01 |
| 卷号 | 412 |
| 关键词 | SERS DNAzyme Fe3O4@Au@Ag NPs Lead ions Human serum |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-020-02709-2 |
| 通讯作者 | Zhao, Aiwu(awzhao@iim.ac.cn) |
| 英文摘要 | Lead ions (Pb2+), one form of the toxic heavy metal, have drawn significant attention due to their harmful effects on human health and the environment. Although many analytical techniques have been developed over the past few decades, the development of a sensitive, selective, and rapid method to detect Pb(2+)remains a challenge. In this work, we developed a sensitive surface-enhanced Raman scattering (SERS) biosensor for highly sensitive detection of Pb(2+)by using DNAzyme-modified Fe3O4@Au@Ag nanoparticles (Fe3O4@Au@Ag NPs). Firstly, the thiolated 5'-Cy3 DNA probe was modified on the surface of Fe3O4@Au@Ag NPs, which hybridized with the Pb2+-specific DNAzyme to form a SERS biosensor, and the Cy3 labels were used to detect Pb2+. In the presence of Pb2+, the DNAzyme cleaves the Cy3-labeled DNA probe, leading to the release of Cy3-labeled DNA probe from the Fe3O4@Au@Ag NPs. Therefore, the Raman intensity of the Cy3 labels decreases. The proposed biosensor exhibited excellent linearity in the range from 0.01 to 1.0 nM, with a limit of detection for Pb(2+)of 5 pM. It features superior selectivity to Pb(2+)over other interfering metal ions and good application in the determination of Pb(2+)in tap water and human serum samples. The SERS biosensor provides a novel, simple and sensitive method for detection of Pb(2+)and sheds new light on the design and synthesis of analogous SERS biosensors for the detection of other heavy metal ions. |
| WOS关键词 | ENHANCED RAMAN-SCATTERING ; ULTRASENSITIVE DETECTION ; PB2+ ; SENSOR ; PLATFORM ; CADMIUM ; COPPER ; PROBE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000544691400010 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/71028]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhao, Aiwu |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China 4.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 5.Cent South Univ, Xiangya Hosp 3, Clin Lab, Changsha 410013, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Wei,Zhao, Aiwu,Zu, Fangtao,et al. A highly sensitive DNAzyme-based SERS biosensor for quantitative detection of lead ions in human serum[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2020,412. |
| APA | Xu, Wei,Zhao, Aiwu,Zu, Fangtao,Khan, Ranjha,Hussain, Hafiz Muhammad Jafar,&Li, Jian.(2020).A highly sensitive DNAzyme-based SERS biosensor for quantitative detection of lead ions in human serum.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,412. |
| MLA | Xu, Wei,et al."A highly sensitive DNAzyme-based SERS biosensor for quantitative detection of lead ions in human serum".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 412(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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