Cell Locating with the Image Analysis System of the CAS-LIBB Single-Particle Microbeara Facility*
文献类型:期刊论文
作者 | Wang Xiaohua1; Wang Shaohu1; Yu Zengliang1 |
刊名 | Plasma Science & Technology
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出版日期 | 2005 |
卷号 | 7 |
关键词 | microbeam irradiation protocol location segmentation |
ISSN号 | 1009-0630 |
其他题名 | Cell Locating with the Image Analysis System of the CAS-LIBB Single-Particle Microbeara Facility* |
英文摘要 | A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). At the CAS-LIBB mi-crobeam facility, we have developed protocols to place exact numbers of charged particles through nuclear centroids of cells, at defined positions in the cytoplasm relative to the nucleus, and through denned fractions of cells in a population. In this paper, we address the methods for nucleus, cyto-plasm and bystander (either a single or an exact number of ions is delivered to a certain percentage of cells in a population to study the bystander effects of radiation) irradiation in detail from the precision of target finding and cell locating in the image analysis system. Moreover, for cells touching slightly in an image, a watershed method is used to separate these touching objects; after that, the number of objects in an image is counted accurately and the irradiation points are located precisely. |
语种 | 英语 |
CSCD记录号 | CSCD:1998387 |
源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/94467] ![]() |
专题 | 中国科学院合肥物质科学研究院 |
作者单位 | 1.Institute of Plasma Physics, Chinese Academy of Sciences 2.Institute of Plasma Physics, Chinese Academy of Sciences 3.Institute of Plasma Physics, Chinese Academy of Sciences |
推荐引用方式 GB/T 7714 | Wang Xiaohua,Wang Shaohu,Yu Zengliang. Cell Locating with the Image Analysis System of the CAS-LIBB Single-Particle Microbeara Facility*[J]. Plasma Science & Technology,2005,7. |
APA | Wang Xiaohua,Wang Shaohu,&Yu Zengliang.(2005).Cell Locating with the Image Analysis System of the CAS-LIBB Single-Particle Microbeara Facility*.Plasma Science & Technology,7. |
MLA | Wang Xiaohua,et al."Cell Locating with the Image Analysis System of the CAS-LIBB Single-Particle Microbeara Facility*".Plasma Science & Technology 7(2005). |
入库方式: OAI收割
来源:合肥物质科学研究院
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