中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging

文献类型：期刊论文

作者Yang, Guanqing5,6; Liu, Zhengjie5,6; Zhang, Ruilong5,6,7; Tian, Xiaohe5,6; Chen, Juan5,6; Han, Guangmei5,6; Liu, Bianhua1; Han, Xinya2; Fu, Yao4; Hu, Zhangjun3
刊名ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期2020-07-13
关键词imaging probes nucleic acids proteins reactive oxygen species super-resolution microscopy
ISSN号1433-7851
DOI10.1002/anie.202005959
通讯作者Zhang, Ruilong(zrl@ahu.edu.cn) ; Zhang, Zhongping(zpzhang@iim.ac.cn)
英文摘要Understanding the biomolecular interactions in a specific organelle has been a long-standing challenge because it requires super-resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super-resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H(2)O(2)level leads to disassociation of nucleic acid-protein complexes.
WOS关键词HYDROGEN-PEROXIDE ; BINDING ; MICROSCOPY ; TRANSCRIPTION ; REPLICATION ; PROTEINS ; RNA
资助项目National Natural Science Foundation of China[21775001] ; National Natural Science Foundation of China[21705001] ; National Natural Science Foundation of China[21974001] ; National Natural Science Foundation of China[21976183] ; Anhui Provincial Natural Science Foundation of China[1808085MB32] ; Hefei Physical Science Center Foundation[2017FXZY001]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000548161900001
出版者WILEY-V C H VERLAG GMBH
资助机构National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation of China ; Hefei Physical Science Center Foundation
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/94802]  
专题中国科学院合肥物质科学研究院
通讯作者Zhang, Ruilong; Zhang, Zhongping
作者单位1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
2.Anhui Univ Technol, Sch Chem & Chem Engn, Maanshan 243032, Anhui, Peoples R China
3.Linkoping Univ, Dept Phys Chem & Biol, S-58183 Linkoping, Sweden
4.Univ Sci & Technol China, Dept Chem, Hefei 230026, Anhui, Peoples R China
5.Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Anhui, Peoples R China
6.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Anhui, Peoples R China
7.Anhui Univ, Minist Educ, Key Lab Struct & Funct Regulat Hybrid Mat, Hefei 230601, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Guanqing,Liu, Zhengjie,Zhang, Ruilong,et al. A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2020.
APA Yang, Guanqing.,Liu, Zhengjie.,Zhang, Ruilong.,Tian, Xiaohe.,Chen, Juan.,...&Zhang, Zhongping.(2020).A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION.
MLA Yang, Guanqing,et al."A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020).

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。