Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer
文献类型：期刊论文
| 作者 | Li, Jianqiang1,6,7; Li, Xingliang2,3; Wang, Wenxian4,6,7; Shao, Yang5; Zhang, Yiping4,6,7; Song, Zhengbo4,6,7 |
| 刊名 | TRANSLATIONAL ONCOLOGY
 |
| 出版日期 | 2020-08-01 |
| 卷号 | 13 |
| ISSN号 | 1936-5233 |
| DOI | 10.1016/j.tranon.2020.100784 |
| 通讯作者 | Song, Zhengbo(songzb@zjcc.org.cn) |
| 英文摘要 | OBJECTIVE: This study investigated the feasibility of using malignant pleural effusion (MPE) supernatant and paired cell blocks (precipitate) for gene profiling in patients with non-small cell lung cancer (NSCLC) using next-generation sequencing (NGS) technique. METHODS: Stage IV non-squamous NSCLC patients with MPE were eligible in this prospective study and recruited from Zhejiang Cancer Hospital between May 2014 and October 2015. MPE supernatant and paired precipitate sample gene alterations were determined with NGS containing 14 cancer-related genes. Progression free survival (PFS) was evaluated using Kaplan-Meier method and compared using log-rank test. RESULTS: A total of 102 patients were enrolled in the present study. All pleural effusions were confirmed as malignant with cytological smears. A total of 77 paired MPE supernatant and precipitate samples were acquired from the 102 patients. The results revealed that there were no statistically significant differences in the detection rate and maximum allelic fraction between supernatant and precipitate samples (P = 1.0 and P = .6). Collectively, 172 and 158 genomic alterations with 112 shared mutations were identified in supernatant and precipitate samples, respectively. Comparable PFS was found in FGFR mutation patients according to the supernatant and precipitate sample results (14.0 vs.13.9 months, P = .90). CONCLUSIONS: These results demonstrated that MPE supernatants were comparable to precipitate samples for detection of genetic alteration. However, gene mutation heterogeneity was found between these two media types. |
| WOS关键词 | LIQUID BIOPSY ; EGFR MUTATION ; FREE DNA ; CRIZOTINIB ; TISSUE ; NSCLC ; ALK |
| 资助项目 | National Natural Science Foundation of China[81802276] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000544673300003 |
| 出版者 | ELSEVIER SCIENCE INC |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/94808]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Song, Zhengbo |
| 作者单位 | 1.Zhejiang Canc Hosp, Dept Thorac Oncol Surg, Hangzhou 310022, Peoples R China 2.Zhejiang Rongjun Hosp, Dept Thorac Dis Diag, Jiaxing 314000, Zhejiang, Peoples R China 3.Zhejiang Rongjun Hosp, Treatment Ctr, Jiaxing 314000, Zhejiang, Peoples R China 4.Zhejiang Canc Hosp, Dept Med Oncol, Hangzhou 301122, Zhejiang, Peoples R China 5.Geneseeq Technol Inc, Translat Med Res Ctr, Toronto, ON, Canada 6.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Peoples R China 7.Univ Chinese Acad Sci, Dept Thorac Oncol Surg, Canc Hosp, Hangzhou 310022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jianqiang,Li, Xingliang,Wang, Wenxian,et al. Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer[J]. TRANSLATIONAL ONCOLOGY,2020,13. |
| APA | Li, Jianqiang,Li, Xingliang,Wang, Wenxian,Shao, Yang,Zhang, Yiping,&Song, Zhengbo.(2020).Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer.TRANSLATIONAL ONCOLOGY,13. |
| MLA | Li, Jianqiang,et al."Gene Alterations in Paired Supernatants and Precipitates from Malignant Pleural Effusions of Non-Squamous Non-Small Cell Lung Cancer".TRANSLATIONAL ONCOLOGY 13(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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