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Raman micro-spectroscopy monitoring of cytochrome c redox state in Candida utilis during cell death under low-temperature plasma-induced oxidative stress

文献类型：期刊论文

作者Chen, Zhu2,3; Liu, Jinghua1,2,3; Tian, Lijiao2; Zhang, Qianqian2,3; Guan, Yong2; Chen, Liang2; Liu, Gang2; Yu, Han-qing2; Tian, Yangchao2; Huang, Qing2,3
刊名ANALYST
出版日期2020-06-07
卷号145
ISSN号0003-2654
DOI10.1039/d0an00507j
通讯作者Huang, Qing(huangq@ipp.ac.cn)
英文摘要Oxidative stress may result in different modes of cell death, such as necrosis, apoptosis and necroptosis. Currently, researchers are still striving to develop efficient tools/methods to distinguish the cell death modes in direct and label-free ways. In this study, we attempted to employ Raman micro-spectroscopy to observe the molecular changes in Candida utilis cells under oxidative stress induced by low-temperature plasma (LTP) and explore the spectroscopic biomarkers for the modes of cell death under oxidative stress. In this research, we confirmed that LTP could impose oxidative stress on the yeast cells, and recorded the changes of Raman signals of cytochrome c in the cells under LTP oxidative stress. Subsequently, we identified the biochemical and morphological characteristic features corresponding to different modes of cell death. Interestingly, we found that LTP under certain conditions could induce oxidative stress which caused the yeast cell death mainly by means of necroptosis, which was verified by Annexin V/PI, HMGB1 location assay and immunoprecipitation assay of the RIP1/RIP3 necrosome. Correspondingly, we also showed that the LTP induced necroptosis, associated with the increase of cytoplasmic Ca2+ and mitochondrial ROS, the decrease of mitochondrial membrane potential, the release of oxidized cytochrome c from the mitochondrion to the cytoplasm, and the destruction of mitochondria in yeast cells. This work has therefore demonstrated that monitoring the redox state of cytochrome c using Raman micro-spectroscopy is very useful for distinguishing the modes of cell death and particularly may unveil the unique necroptosis process of cells under extrinsic oxidative stress.
WOS关键词IN-VIVO ; NECROPTOSIS ; MITOCHONDRIA ; ACTIVATION ; PATHWAYS ; NECROSIS ; RELEASE ; MARKER
资助项目National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11775272] ; National Natural Science Foundation of China[11475217]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000539274100016
出版者ROYAL SOC CHEMISTRY
资助机构National Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/102997]  
专题中国科学院合肥物质科学研究院
通讯作者Huang, Qing
作者单位1.Anhui Sci & Technol Univ, Coll Life Sci, Fengyang 233100, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Zhu,Liu, Jinghua,Tian, Lijiao,et al. Raman micro-spectroscopy monitoring of cytochrome c redox state in Candida utilis during cell death under low-temperature plasma-induced oxidative stress[J]. ANALYST,2020,145.
APA Chen, Zhu.,Liu, Jinghua.,Tian, Lijiao.,Zhang, Qianqian.,Guan, Yong.,...&Huang, Qing.(2020).Raman micro-spectroscopy monitoring of cytochrome c redox state in Candida utilis during cell death under low-temperature plasma-induced oxidative stress.ANALYST,145.
MLA Chen, Zhu,et al."Raman micro-spectroscopy monitoring of cytochrome c redox state in Candida utilis during cell death under low-temperature plasma-induced oxidative stress".ANALYST 145(2020).

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来源：合肥物质科学研究院

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