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A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection

文献类型：期刊论文

作者Gao, Qingxue3,4; Chang, Yongjia4; Deng, Qingmei2; You, Hui1
刊名ANALYTICAL METHODS
出版日期2020-05-28
卷号12
ISSN号1759-9660
DOI10.1039/d0ay00240b
通讯作者You, Hui(usmlhy@iim.ac.cn)
英文摘要Blood plasma separation is a vital sample pre-treatment procedure for microfluidic devices in blood diagnostics, and it requires reliability and speediness. In this work, we propose a novel and simple method for microvolume blood plasma separation driven by capillary force. Flat-shaped filter membranes combined with hydrophilic narrow capillaries are introduced into devices, in order to reduce the residual volumes of blood plasma. An interference fit is used to ensure no leakage of blood or cells. There is desired trapping efficiency of blood cells in the devices. The method provides high efficiency with a plasma extraction yield of 71.7% within 6 min, using 60 mu L of undiluted whole human blood with 45% haematocrit. The influence from structural parameters on the separation kinetics and the dependence of the haematocrit levels on the separation efficiency are also investigated. The total protein detection shows considerable protein recovery of 82.3% in the extracted plasma. Thus, the plasma separation unit with a very simple structure is suitable for integrating into microfluidic devices, presenting promising prospects for clinical diagnostics as well as for point-of-care testing applications.
WOS关键词WHOLE-BLOOD ; HEMOLYSIS ; CHIP ; FILTER
资助项目Key Research and Development Plan Project of Anhui Province[1704a0802157] ; Key-Area Research and Development Program of Guangdong Province[2019B020219003]
WOS研究方向Chemistry ; Food Science & Technology ; Spectroscopy
语种英语
WOS记录号WOS:000537867200003
出版者ROYAL SOC CHEMISTRY
资助机构Key Research and Development Plan Project of Anhui Province ; Key-Area Research and Development Program of Guangdong Province
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/103077]  
专题中国科学院合肥物质科学研究院
通讯作者You, Hui
作者单位1.Guangxi Univ, Sch Mech Engn, Nanning 530004, Guangxi, Peoples R China
2.Canc Hosp, Chinese Acad Sci, Dept Lab, Hefei 230031, Anhui, Peoples R China
3.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
4.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Qingxue,Chang, Yongjia,Deng, Qingmei,et al. A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection[J]. ANALYTICAL METHODS,2020,12.
APA Gao, Qingxue,Chang, Yongjia,Deng, Qingmei,&You, Hui.(2020).A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection.ANALYTICAL METHODS,12.
MLA Gao, Qingxue,et al."A simple and rapid method for blood plasma separation driven by capillary force with an application in protein detection".ANALYTICAL METHODS 12(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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