Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach
文献类型：期刊论文
| 作者 | Sun, Yang1,2; Gao, Fei1,2; Yang, Cai1,2; Li, Yingying4,5 ; Jin, Cheng4,5; Xie, Sitao4,5; Lv, Cheng2; Ding, Ding2; Han, Da2; Li, Juan2
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| 刊名 | BIOCONJUGATE CHEMISTRY
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| 出版日期 | 2020-05-01 |
| 卷号 | 31 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/acs.bioconjchem.0c00071 |
| 通讯作者 | Wang, Ruowen(wangwrw@sjtu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn) |
| 英文摘要 | Bispecific aptamer-drug conjugates (BsApDC) may improve the efficacy of drugs by enhancing cellular internalization and targeted delivery. Nevertheless, the synthesis of single-molecular BsApDC has not yet been reported, and it could be thwarted by synthetic challenges. Herein we report a general approach to synthesize a BsApDC hybridized chemical and biological method. Primers incorporated with 5-Fluorouracil (5-FU), 10-Hydroxycamptothecin, and Maleimidocaproyl-valinecitrulline-p-aminobenzoyloxycarbonyl-monomethyl auristatin E(vcMMAE) were prepared by chemical synthesis, which were converted to corresponding ApDCs efficiently by enzymatic reaction. Biological studies revealed that BsApDC binds with target cells with enhanced internalization and better inhibitory activity, demonstrating the potential of BsApDCs for targeted tumor therapy. |
| WOS关键词 | NEXT-GENERATION ; DNA ; DELIVERY ; GROWTH ; CELLS |
| 资助项目 | NSFC[NSFC 21877079] ; NSFC[NSFC 81803068] ; ZJNSF[LY16B020001] ; NIH[GM R35 127130] ; NSF[1645215] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000537398400008 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | NSFC ; ZJNSF ; NIH ; NSF |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/103105]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wang, Ruowen; Tan, Weihong |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Coll Chem & Chem Engn, Shanghai 200240, Peoples R China 2.Shanghai Jiao Tong Univ, Renji Hosp, Inst Mol Med, Sch Med,State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China 3.Univ Chinese Acad Sci, Chinese Acad Sci, Canc Hosp, Inst Canc & Basic Med IBMC, Hangzhou 310022, Zhejiang, Peoples R China 4.Hunan Univ, Aptamer Engn Ctr Hunan Prov, Changsha 410082, Hunan, Peoples R China 5.Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn,Mol Sci & Biomed Lab MBL, Changsha 410082, Hunan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yang,Gao, Fei,Yang, Cai,et al. Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach[J]. BIOCONJUGATE CHEMISTRY,2020,31. |
| APA | Sun, Yang.,Gao, Fei.,Yang, Cai.,Li, Yingying.,Jin, Cheng.,...&Tan, Weihong.(2020).Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach.BIOCONJUGATE CHEMISTRY,31. |
| MLA | Sun, Yang,et al."Construction of Bispecific Aptamer-Drug Conjugate by a Hybrid Chemical and Biological Approach".BIOCONJUGATE CHEMISTRY 31(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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