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An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome

文献类型：期刊论文

作者Yang, Linlin1,3; Zhao, Jun1,3; Wang, Jianping1,3; Han, Guangmei1,2,3; Liu, Bianhua1,3; Zhang, Wei4; Fu, Yao5; Han, Ming-Yong1,3; Wang, Zhenyang1,3; Zhang, Zhongping1,2,3
刊名TALANTA
出版日期2020-08-15
卷号216
ISSN号0039-9140
关键词Fluorescent probe Lysosome Autolysosome Azacyclo localization Cell imaging
DOI10.1016/j.talanta.2020.120941
通讯作者Han, Guangmei(gmhan@iim.ac.cn) ; Wang, Zhenyang(zywang@iim.ac.cn)
英文摘要Understanding lysosome-related physiology needs specific lysosome probes to track the biological processes of lysosome in living cells. Here, we report an azacyclo-modified fluorescent probe that has a large Stokes shift, good photostability and negligible cytotoxicity for highly specific labeling of lysosome and autolysosome in living cells. The probes with different kinds of azacyclo groups on parent dye dansyl are screened to show that dansyl-cycleanine (DNS-C) with four nitrogen atoms possesses the best lysosome-localized ability. And DNS-C as a universal tracker exhibits excellent ability for lysosome labeling in different cell lines with high overlap coefficients (>= 0.90). Different from a commercially available LysoTracker, the Stokes shift of DNS-C up to 240 nm (lambda(ex/em) = 330/570 nm), is much larger than that of LysoTracker similar to 20 nm (lambda(ex/em) = 573/595 nm). More importantly, the fluorescence of DNS-C keeps still high brightness after a time-lapsed imaging for 40 min in living cells, implying its remarkable photostability for long-term tracking. In addition, DNS-C can also clearly image the autolysosome, a critical subcellular compartment, forming by the fusion of lysosome with autophagosome in autophagy. These results suggest the promising utility of our probe as a powerful tool to real-time trace physiological processes of lysosomes.
WOS关键词AUTOPHAGY ; PH ; TRACKING ; ENZYMES ; CARBON ; ACID
资助项目National Natural Science Foundation of China[61705239] ; National Natural Science Foundation of China[21605145] ; National Natural Science Foundation of China[21775001] ; National Natural Science Foundation of China[21806168] ; National Natural Science Foundation of China[21874137] ; National Natural Science Foundation of China[21976183] ; Hefei Physical Science Center Foundation[2017FXZY001]
WOS研究方向Chemistry
语种英语
出版者ELSEVIER
WOS记录号WOS:000536207600014
资助机构National Natural Science Foundation of China ; Hefei Physical Science Center Foundation
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/103133]  
专题中国科学院合肥物质科学研究院
通讯作者Han, Guangmei; Wang, Zhenyang
作者单位1.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China
2.Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Peoples R China
3.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China
4.Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Shandong, Peoples R China
5.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Linlin,Zhao, Jun,Wang, Jianping,et al. An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome[J]. TALANTA,2020,216.
APA Yang, Linlin.,Zhao, Jun.,Wang, Jianping.,Han, Guangmei.,Liu, Bianhua.,...&Zhang, Zhongping.(2020).An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome.TALANTA,216.
MLA Yang, Linlin,et al."An azacyclo-localizing fluorescent probe for the specific labeling of lysosome and autolysosome".TALANTA 216(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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