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Construction of a human cell landscape at single-cell level

文献类型：期刊论文

作者Han, Xiaoping17,18; Zhou, Ziming17; Fei, Lijiang17; Sun, Huiyu17; Wang, Renying17; Chen, Yao1; Chen, Haide2,17; Wang, Jingjing2,17; Tang, Huanna3; Ge, Wenhao4
刊名NATURE
出版日期2020-05-01
卷号581
ISSN号0028-0836
DOI10.1038/s41586-020-2157-4
通讯作者Han, Xiaoping(xhan@zju.edu.cn) ; Guo, Guoji(ggj@zju.edu.cn)
英文摘要Single-cell analysis is a valuable tool for dissecting cellular heterogeneity in complex systems(1). However, a comprehensive single-cell atlas has not been achieved for humans. Here we use single-cell mRNA sequencing to determine the cell-type composition of all major human organs and construct a scheme for the human cell landscape (HCL). We have uncovered a single-cell hierarchy for many tissues that have not been well characterized. We established a 'single-cell HCL analysis' pipeline that helps to define human cell identity. Finally, we performed a single-cell comparative analysis of landscapes from human and mouse to identify conserved genetic networks. We found that stem and progenitor cells exhibit strong transcriptomic stochasticity, whereas differentiated cells are more distinct. Our results provide a useful resource for the study of human biology. Single-cell RNA sequencing is used to generate a dataset covering all major human organs in both adult and fetal stages, enabling comparison with similar datasets for mouse tissues.
WOS关键词MESSENGER-RNA-SEQ ; REVEALS ; MOUSE ; EXPRESSION ; IDENTITY ; RECONSTRUCTION ; DIVERSITY ; ATLAS
资助项目National Natural Science Foundation of China[91842301] ; National Natural Science Foundation of China[81770188] ; National Natural Science Foundation of China[31722027] ; National Natural Science Foundation of China[31922049] ; National Natural Science Foundation of China[31701290] ; National Natural Science Foundation of China[31871473] ; National Key Research and Development Program[2018YFA0107804] ; National Key Research and Development Program[2018YFA0107801] ; National Key Research and Development Program[2018YFA0800503] ; National Key Research and Development Program[2018YFC1005003] ; Zhejiang Provincial Natural Science Foundation of China[R17H080001] ; Fundamental Research Funds for the Central Universities
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000532687800001
出版者NATURE PUBLISHING GROUP
资助机构National Natural Science Foundation of China ; National Key Research and Development Program ; Zhejiang Provincial Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/103247]  
专题中国科学院合肥物质科学研究院
通讯作者Han, Xiaoping; Guo, Guoji
作者单位1.Zhejiang Univ, Womens Hosp, Sch Med, Dept Reprod Endocrinol, Hangzhou, Peoples R China
2.Dr Li Dak Sum & Yip Yio Chin Ctr Stem Cell & Rege, Zhejiang Prov Key Lab Tissue Engn & Regenerat Med, Hangzhou, Peoples R China
3.Zhejiang Univ, Affiliated Hosp 1, Kidney Dis Ctr, Sch Med, Hangzhou, Peoples R China
4.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Hepatobiliary & Pancreat Surg, Hangzhou, Peoples R China
5.Zhejiang Univ, Coll Life Sci, Hangzhou, Peoples R China
6.Zhejiang Univ, Ctr Neurosci, Sch Med, Hangzhou, Peoples R China
7.Zhejiang Univ, Affiliated Hosp 1, Sch Med, Dept Hepatobiliary & Pancreat Surg, Hangzhou, Peoples R China
8.Hangzhou Repugene Technol, Hangzhou, Peoples R China
9.Zhejiang Univ, Stem Cell Inst, Hangzhou, Peoples R China
10.Zhejiang Univ, Inst Hematol, Hangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Han, Xiaoping,Zhou, Ziming,Fei, Lijiang,et al. Construction of a human cell landscape at single-cell level[J]. NATURE,2020,581.
APA Han, Xiaoping.,Zhou, Ziming.,Fei, Lijiang.,Sun, Huiyu.,Wang, Renying.,...&Guo, Guoji.(2020).Construction of a human cell landscape at single-cell level.NATURE,581.
MLA Han, Xiaoping,et al."Construction of a human cell landscape at single-cell level".NATURE 581(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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