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Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ

文献类型：期刊论文

作者Ni, Wenfeng1,2; Zheng, Zhiming1; Liu, Hui1; Wang, Peng1; Wang, Li1; Wang, Han1,2; Sun, Xiaowen1,2; Yang, Qiang1,2; Tang, Hengfang1,2; Zhao, Genhai1
刊名CARBOHYDRATE POLYMERS
出版日期2020-05-01
卷号235
关键词Carboxymethyl cellulose Magnetic nanoparticles Prenyltransferase NovQ Immobilization Prenylated activity
ISSN号0144-8617
DOI10.1016/j.carbpol.2020.115955
通讯作者Zheng, Zhiming(zhengzhiming2014@163.com) ; Zhao, Genhai(zhgh327@126.com)
英文摘要Prenyltransferase NovQ immobilized carboxymethyl cellulose magnetic nanoparticles (NCMNs) were successfully synthesized via a valuable approach integrated from nanocomposite preparation, and applied for the production of vitamin K-2 using menadione hydroquinol and dimethylallyl diphosphate (DMAPP) as substrates. To investigate the interaction between nanoparticles and NovQ, we characterized the nanocomposite, and revealed that carboxymethyl cellulose (CMC) and Fe3O4 formed a core-shell structure to absorb NovQ in the reaction systems, resulting from the high affinity of immobilized materials. Meanwhile, NCMNs with excellent pH and temperature tolerance, enhanced prenylated activity, and improved stability were found. Molecular docking analysis was also conducted to justify the contribution of multiple amino acids and effect of nanoparticles on catalytic properties of NovQ. Taken together, our study introduces a promising strategy to prepare magnetic nanoparticles and improve the performance of catalyst, which aims for opening new orientations for synthesis of magnetic nanoparticles used for prenyltransferase immobilization.
WOS关键词FE3O4 NANOPARTICLES ; LIPASE ; PURIFICATION ; BIOCATALYST ; ENZYMES
资助项目Key research and development plan of Anhui Province[1804b06020342] ; Natural Science Foundation of Anhui Province[1908085MB48] ; Natural Science Foundation of Anhui Province[1908085MB43] ; China National Key Research and Development Program[2019YFA0904300] ; Major Projects of Science and Technology in Anhui Province[17030801036]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000519044900022
出版者ELSEVIER SCI LTD
资助机构Key research and development plan of Anhui Province ; Natural Science Foundation of Anhui Province ; China National Key Research and Development Program ; Major Projects of Science and Technology in Anhui Province
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/103645]  
专题中国科学院合肥物质科学研究院
通讯作者Zheng, Zhiming; Zhao, Genhai
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Comprehens Lab Bldg,350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Ni, Wenfeng,Zheng, Zhiming,Liu, Hui,et al. Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ[J]. CARBOHYDRATE POLYMERS,2020,235.
APA Ni, Wenfeng.,Zheng, Zhiming.,Liu, Hui.,Wang, Peng.,Wang, Li.,...&Zhao, Genhai.(2020).Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ.CARBOHYDRATE POLYMERS,235.
MLA Ni, Wenfeng,et al."Synthesis of the carboxymethyl cellulose magnetic nanoparticles for efficient immobilization of prenyltransferase NovQ".CARBOHYDRATE POLYMERS 235(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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