Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway
文献类型：期刊论文
| 作者 | Xu, Shenglin4; Wang, Yuan4; Que, Yukang4; Ma, Chengxiao4; Cai, Shang4; Wang, Hao4 ; Yang, Xingyu1; Yang, Chunjun1; Cheng, Cheng2; Zhao, Guoping3
|
| 刊名 | ONCOLOGY REPORTS
 |
| 出版日期 | 2020-05-01 |
| 卷号 | 43 |
| 关键词 | cold atmospheric plasma osteosarcoma mitochondrial reactive oxygen species apoptosis |
| ISSN号 | 1021-335X |
| DOI | 10.3892/or.2020.7518 |
| 通讯作者 | Hu, Yong(hy.in163@163.com) |
| 英文摘要 | The present study aimed to investigate the effects of cold atmospheric plasma (CAP)-activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP-activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin-V and propidium iodide. The expression levels of cytochrome c, caspase-3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC-1 dye method and verified by the level of reactive oxygen species (ROS). CAP-activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose- and time-dependent manner. Furthermore, CAP-activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP-activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS-mediated mitochondrial apoptosis. |
| WOS关键词 | PROGNOSTIC-FACTORS ; SURVIVAL ; CHEMOTHERAPY ; EXPERIENCE ; CHILDREN ; MEDIA ; HEAD ; PART |
| 资助项目 | Natural Science Foundation Project of Anhui Province[1708085MH215] ; School Fund of Anhui Medical University[2015xkj088] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000526743200029 |
| 出版者 | SPANDIDOS PUBL LTD |
| 资助机构 | Natural Science Foundation Project of Anhui Province ; School Fund of Anhui Medical University |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/103655]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Hu, Yong |
| 作者单位 | 1.Anhui Med Univ, Dept Dermatol, Affiliated Hosp 2, Hefei 230000, Anhui, Peoples R China 2.Chinese Acad Sci, Inst Plasma Phys, Hefei 230000, Anhui, Peoples R China 3.Chinese Acad Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, Hefei Inst Phys Sci, Hefei 230000, Anhui, Peoples R China 4.Anhui Med Univ, Dept Orthoped, Affiliated Hosp 1, 218 Jixi Rd, Hefei 230000, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Shenglin,Wang, Yuan,Que, Yukang,et al. Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway[J]. ONCOLOGY REPORTS,2020,43. |
| APA | Xu, Shenglin.,Wang, Yuan.,Que, Yukang.,Ma, Chengxiao.,Cai, Shang.,...&Hu, Yong.(2020).Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway.ONCOLOGY REPORTS,43. |
| MLA | Xu, Shenglin,et al."Cold atmospheric plasma-activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway".ONCOLOGY REPORTS 43(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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