Genotoxicity of microcystin-LR in mammalian cells: Implication from peroxynitrite produced by mitochondria
文献类型：期刊论文
| 作者 | Wang, Xiaofei2,3 ; Li, Yintao3; Xiao, Hourong3; Zhang, Min3; Bao, Teng3; Luo, Xun1; Chen, Shaopeng2
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| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2020-06-01 |
| 卷号 | 195 |
| 关键词 | Microcystin-LR DNA damage Peroxynitrite Mitochondria |
| ISSN号 | 0147-6513 |
| DOI | 10.1016/j.ecoenv.2020.110408 |
| 通讯作者 | Wang, Xiaofei(wxf1985@mail.ustc.edu.cn) ; Chen, Shaopeng(spchen035@ipp.ac.cn) |
| 英文摘要 | Microcystin-LR (MC-LR) is a widely known hepatotoxin which could induce the occurrence and metastasis of hepatocellular carcinoma. In recent years, with the frequent outbreak of cyanobacteria, the harm of MC-LR has gradually attracted more attention. Hence, this study focused on the effect of MC-LR on DNA damage in HepG2 cells, identifying the types and sources of free radicals that make an important function on this issue. Our data suggested that MC-LR induced concentration- and time-dependent increasement of DNA double-strand breaks (DSBs). After exposure to 1 mu M MC-LR for 3 days, the protein expression and immunofluorescence staining of gamma-H2AX was significantly increased. Using a scavenger of mitochondrial O-2(center dot-)(4-hydroxy-tempo), a inhibitor of mitochondrial NOS (7-nitroindazole), and a scavenger of ONOO- (uric acid), it was revealed that ONOO- originated from mitochondria made a significant contribution to the genotoxicity of MC-LR. Moreover, a significant decreasement of mitochondrial membrane potential (MMP) was observed. These findings suggested that peroxynitrite targeting mitochondria plays a vital role in the MC-LR-induced genotoxic response in mammalian cells. |
| WOS关键词 | OXIDATIVE STRESS ; CHRONIC EXPOSURE ; HISTONE H2AX ; NITRIC-OXIDE ; HEPG2 CELLS ; DNA-DAMAGE ; CYTOTOXICITY ; DYSFUNCTION ; APOPTOSIS ; LIVER |
| 资助项目 | Natural Science Foundation of Anhui Province[1808085QB37] ; Opening Foundation of Anhui Province of Key Laboratory of Environmental Toxicology and Pollution Control Technology[Y79AH72588-02] ; Talent Research Foundation of Hefei University[16-17RC03] ; Key Project of Natural Science Research of Anhui High Education Institutions[KJ 2018A0561] ; Key Project of Natural Science Research of Anhui High Education Institutions[KJ20180471] ; Major Project of Research Foundation of Hefei University[17ZR08ZDA] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000525762800024 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | Natural Science Foundation of Anhui Province ; Opening Foundation of Anhui Province of Key Laboratory of Environmental Toxicology and Pollution Control Technology ; Talent Research Foundation of Hefei University ; Key Project of Natural Science Research of Anhui High Education Institutions ; Major Project of Research Foundation of Hefei University |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/103665]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wang, Xiaofei; Chen, Shaopeng |
| 作者单位 | 1.Huainan Normal Univ, Sch Biol Engn, Huainan 232001, Peoples R China 2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Prov Key Lab Environm Toxicol & Pollut Cont, Hefei 230031, Peoples R China 3.Hefei Univ, Sch Biol Food & Environm, Hefei 230601, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaofei,Li, Yintao,Xiao, Hourong,et al. Genotoxicity of microcystin-LR in mammalian cells: Implication from peroxynitrite produced by mitochondria[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,195. |
| APA | Wang, Xiaofei.,Li, Yintao.,Xiao, Hourong.,Zhang, Min.,Bao, Teng.,...&Chen, Shaopeng.(2020).Genotoxicity of microcystin-LR in mammalian cells: Implication from peroxynitrite produced by mitochondria.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,195. |
| MLA | Wang, Xiaofei,et al."Genotoxicity of microcystin-LR in mammalian cells: Implication from peroxynitrite produced by mitochondria".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 195(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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