Magnetic quantum dot based lateral flow assay biosensor for multiplex and sensitive detection of protein toxins in food samples
文献类型：期刊论文
| 作者 | Wang, Chongwen1,2,5; Xiao, Rui2,5; Wang, Shu1,4; Yang, Xingsheng1; Bai, Zikun5; Li, Xinying3; Rong, Zhen2,5; Shen, Beifen3; Wang, Shengqi1,2,5 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2019-12-15 |
| 卷号 | 146 |
| 关键词 | Magnetic-quantum dot nanoparticles Fluorescent lateral flow immunoassay Botulinum neurotoxin type A Staphylococcal enterotoxin Simultaneous detection |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2019.111754 |
| 通讯作者 | Rong, Zhen(rongzhen0525@sina.com) ; Shen, Beifen(shenbf@mx.cei.gov.cn) ; Wang, Shengqi(sqwang@bmi.ac.cn) |
| 英文摘要 | Protein toxins, such as botulinum neurotoxin type A (BoNT/A) and staphylococcal enterotoxin B (SEB), easily pollute food and water and are ultra-toxic to humans and animals, thus requiring a sensitive on-site detection method. In this study, we reported a novel lateral flow assay (LFA) strip on the basis of magnetic quantum dot nanoparticles (MagQD NPs) for sensitive and multiplex protein toxin detection in food samples. A new type of MagQD NP was prepared by fixing the dense carboxylated QDs on the surface of polyethyleneimine-modified Fe3O4 magnetic NPs (MNPs) and applied in LFA with the following functions: capture and enrich target toxins from sample solutions and serve as advanced fluorescent labels for the quantitative determination of targets on the strip. Through this strategy, the assay realized quantified BoNT/A and SEB detection in 30 min with the limits of detection of 2.52 and 2.86 pg/mL, respectively. The selectivity and the ability of quantitative analysis of the method were validated in real food samples, including milk and juice. This MagQD-LFA biosensor showed considerable potential as a point-of-care testing tool for the sensitive detection of trace toxins. |
| WOS关键词 | ENHANCED RAMAN-SPECTROSCOPY ; OF-CARE DETECTION ; ULTRASENSITIVE DETECTION ; MASS-SPECTROMETRY ; IMMUNOASSAY ; DIAGNOSIS ; PLATFORM ; STRIP ; ELISA |
| 资助项目 | National S&T Major Project for Infectious Diseases Control[2018ZX10712001-010] ; National S&T Major Project for Infectious Diseases Control[2018ZX10101003-001] ; National Natural Science Foundation of China[81830101] ; Natural Science Foundation of Anhui Province[1908085QB85] |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000517963500001 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 资助机构 | National S&T Major Project for Infectious Diseases Control ; National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/103941]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Rong, Zhen; Shen, Beifen; Wang, Shengqi |
| 作者单位 | 1.Anhui Agr Univ, Coll Life Sci, Hefei 230036, Peoples R China 2.Beijing Key Lab New Mol Diag Technol Infect Dis, Beijing 100850, Peoples R China 3.Inst Basic Med Sci, Beijing 100850, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei, Peoples R China 5.Beijing Inst Radiat Med, Beijing 100850, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chongwen,Xiao, Rui,Wang, Shu,et al. Magnetic quantum dot based lateral flow assay biosensor for multiplex and sensitive detection of protein toxins in food samples[J]. BIOSENSORS & BIOELECTRONICS,2019,146. |
| APA | Wang, Chongwen.,Xiao, Rui.,Wang, Shu.,Yang, Xingsheng.,Bai, Zikun.,...&Wang, Shengqi.(2019).Magnetic quantum dot based lateral flow assay biosensor for multiplex and sensitive detection of protein toxins in food samples.BIOSENSORS & BIOELECTRONICS,146. |
| MLA | Wang, Chongwen,et al."Magnetic quantum dot based lateral flow assay biosensor for multiplex and sensitive detection of protein toxins in food samples".BIOSENSORS & BIOELECTRONICS 146(2019). |
入库方式： OAI收割
来源：合肥物质科学研究院
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