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Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells

文献类型：期刊论文

作者Chen, Xigao1,2,3,4; Qiu, Liping5; Cai, Ren1,2,3,4; Cui, Cheng1,2,3,4; Li, Long1,2,3,4; Jiang, Jian-hui5; Tan, Weihong1,2,3,4,5,6,7
刊名ACS APPLIED MATERIALS & INTERFACES
出版日期2020-08-26
卷号12
关键词aptamer-directed modification living cell membrane protein multiple labeling protein modification bioorthogonal reactions
ISSN号1944-8244
DOI10.1021/acsami.0c07004
通讯作者Jiang, Jian-hui(jianhuijiang@hnu.edu.cn) ; Tan, Weihong(tan@hnu.edu.cn)
英文摘要Understanding how a cell membrane protein functions on living cells remains a challenge for cell biology. Specific placement of functional molecules on specific proteins in their native environment would allow comprehensive study of proteins' dynamic functions. Existing methods cannot facilely achieve multiple modifications on specific membrane proteins. In this report, we describe an aptamer-induced, protein-specific bio-orthogonal modification technology for precise nongenetic immobilization of multiple small functional molecules on target membrane glycoproteins by combining metabolic technology and aptamer targeting. In brief, DNA probes were designed by modifying aptamers, which bind to target proteins on the surfaces of living cells pretreated with N-azidoacetylmannosamine-tetraacy-lated (Ac(4)ManNAz). The cyclooctynes tagged of DNA probes will approach the azide groups to trigger the bio-orthogonal reactions. After UV irradiation and hybridization with cDNA (complementary DNA), the aptamers can be removed, and the process can be repeated to achieve multiple modifications for multicolor imaging and cell surface nanoengineering on specific proteins.
WOS关键词LIVE CELLS ; ADHESION
资助项目National Institute of General Medical Sciences[GM079359] ; National Cancer Institute[CA133086] ; National Key Scientific Program of China[2011CB911000] ; National Natural Science Foundation of China[21221003] ; National Natural Science Foundation of China[21327009] ; China National Instrumentation Program[2011YQ03012412]
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:000566662000004
出版者AMER CHEMICAL SOC
资助机构National Institute of General Medical Sciences ; National Cancer Institute ; National Key Scientific Program of China ; National Natural Science Foundation of China ; China National Instrumentation Program
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/104018]  
专题中国科学院合肥物质科学研究院
通讯作者Jiang, Jian-hui; Tan, Weihong
作者单位1.Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA
2.Univ Florida, Ctr Res Bio Nano Interface, Dept Chem, Gainesville, FL 32611 USA
3.Univ Florida, Dept Physiol, Gainesville, FL 32611 USA
4.Univ Florida, Funct Genom, Shands Canc Ctr, UF Genet Inst, Gainesville, FL 32611 USA
5.Hunan Univ, Aptamer Engn Ctr Hunan Prov, Mol Sci & Biomed Lab MBL,Coll Biol, State Key Lab Chemo Biosensing & Chemometr,Coll C, Changsha 410082, Hunan, Peoples R China
6.Univ Chinese Acad Sci, Canc Hosp, Hangzhou 310022, Zhejiang, Peoples R China
7.Chinese Acad Sci, Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xigao,Qiu, Liping,Cai, Ren,et al. Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2020,12.
APA Chen, Xigao.,Qiu, Liping.,Cai, Ren.,Cui, Cheng.,Li, Long.,...&Tan, Weihong.(2020).Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells.ACS APPLIED MATERIALS & INTERFACES,12.
MLA Chen, Xigao,et al."Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells".ACS APPLIED MATERIALS & INTERFACES 12(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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