Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins
文献类型：期刊论文
| 作者 | Li, Mengqing1,3; Jia, Jia2,4; Zhang, Xuecheng1,3; Dai, Haiming2,4 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2020-09-10 |
| 卷号 | 530 |
| ISSN号 | 0006-291X |
| 关键词 | Bcl-rambo LC3s/GABARAPs Mitophagy Selective binding |
| DOI | 10.1016/j.bbrc.2020.07.039 |
| 通讯作者 | Zhang, Xuecheng(turenzh@ahu.edu.cn) ; Dai, Haiming(daih@cmpt.ac.cn) |
| 英文摘要 | Mitophagy regulates the metabolic level and cell fates by specifically degrading damaged or redundant mitochondria in these cells. During the formation of autophagosomes, autophagy receptors and adaptors, which usually contain a LC3-interacting region (LIR) domain, are recruited through their interactions with LC3/GABARAP family of proteins. Bcl-rambo is one of the mitophagy receptors that interact with LC3s/GABARAPs. In this study, we first measured the binding of Bcl-rambo to LC3s/GABARAPs in vitro and found Bcl-rambo has a selectivity to LC3C/GABARP/GABARAPL1. Further investigations with bioinfor-matics analyses and mutagenesis suggested that the interactions with the HP1 and HP2 sites of LC3s/GABARAPs and the residues at the X-2 site of the LIR domain of Bcl-rambo are both critical for the selectivity. Moreover, assays in vivo showed that manipulating the selective binding of Bcl-rambo resulted in the changes of mitophagy inductions in the cells. Overall, our data revealed the selective binding between Bcl-rambo and LC3s/GABARAPs and its molecular mechanisms and biological signifi-cances, which will be helpful for future studies of mitophagy mediated by Bcl-rambo. (C) 2020 Elsevier Inc. All rights reserved. |
| WOS关键词 | STRUCTURAL BASIS ; LIR MOTIF ; RECOGNITION ; DEGRADATION ; AUTOPHAGY ; ATG8/LC3 |
| 资助项目 | National Natural Science Foundation of China[21703254] ; National Natural Science Foundation of China[21772201] ; Second Affiliated Hospital of Anhui Medical University ; Center of Medical Physics and Technology[LHJJ202006] ; Center of Medical Physics and Technology[LHJJ202007] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| WOS记录号 | WOS:000567807200021 |
| 资助机构 | National Natural Science Foundation of China ; Second Affiliated Hospital of Anhui Medical University ; Center of Medical Physics and Technology |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/104048]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhang, Xuecheng; Dai, Haiming |
| 作者单位 | 1.Anhui Prov Engn Technol Res Ctr Microorganisms &, Hefei 230601, Anhui, Peoples R China 2.Chinese Acad Sci, Ctr Med Phys & Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China 3.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China 4.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Mengqing,Jia, Jia,Zhang, Xuecheng,et al. Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2020,530. |
| APA | Li, Mengqing,Jia, Jia,Zhang, Xuecheng,&Dai, Haiming.(2020).Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,530. |
| MLA | Li, Mengqing,et al."Selective binding of mitophagy receptor protein Bcl-rambo to LC3/GABARAP family proteins".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 530(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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