Curcumin may reverse 5-fluorouracil resistance on colonic cancer cells by regulating TET1-NKD-Wnt signal pathway to inhibit the EMT progress
文献类型：期刊论文
| 作者 | Lu, Yi5; Zhang, Runzhe4; Zhang, Xinjie4; Zhang, Bo1,2,3; Yao, Qinghua1,2,3 |
| 刊名 | BIOMEDICINE & PHARMACOTHERAPY
 |
| 出版日期 | 2020-09-01 |
| 卷号 | 129 |
| 关键词 | Curcumin Colonic cancer EMT Wnt 5-fluorouracil |
| ISSN号 | 0753-3322 |
| DOI | 10.1016/j.biopha.2020.110381 |
| 通讯作者 | Yao, Qinghua(yaoqh@zjcc.org.cn) |
| 英文摘要 | Background and purpose: Colorectal cancer is a kind of gastrointestinal tumor with rising morbidity and mortality. 5-fluorouracil is one of the most effective chemotherapy drugs for the treatment of CRC. However, clinical data reported dramatic resistance on the treatment for CRC with 5-fluorouracil. Present study aims to explore the anti-resistant effect of curcumin and its mechanism. Methods: MTT assay was used to evaluate the proliferation of rHCT-116 cells. Flow cytometry was used to determine the apoptosis and cell cycle of rHCT-116 cells. Western Blot was performed to detect the expression level of TET1, NKD2, E-cadherin, Vimentin, beta-catenin, TCF4 and Axin in transfected rHCT-116 cells. Results: 5-fluorouracil resistant HCT-116 cells were successfully established. Curcumin was found to be effective in the inhibition of proliferation, inducement of apoptosis and block of G0/G1 phase on 5-fluorouracil treated HCT-116 cells. The expression of TET1 and NKD2 was greatly inhibited by high dosage of curcumin. The WNT signal pathway and EMT progress were suppressed in rHCT-116 cells by high dosage of curcumin. The inhibitory effects of curcumin on WNT signal pathway and EMT progress were verified to be consistent with Pax-6, TET1 and NKD2. Conclusion: Curcumin might exert anti-resistant effect of 5-FU on HCT-116 cells by regulating the TET1-NKD2WNT signal pathway to inhibit the EMT progress. |
| WOS关键词 | EPITHELIAL-MESENCHYMAL TRANSITION ; MULTIDRUG-RESISTANCE ; DOWN-REGULATION ; STEM-CELLS ; TUMOR ; CONTRIBUTES ; EXPRESSION ; GENE ; NKD2 ; STATISTICS |
| 资助项目 | Zhejiang medical and health science and technology program[2016DTA001] |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000566384000001 |
| 出版者 | ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER |
| 资助机构 | Zhejiang medical and health science and technology program |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/104057]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Yao, Qinghua |
| 作者单位 | 1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Key Lab Integrated Tradit Chinese & Western Med, Canc Hosp, 1 Banshandong Rd, Hangzhou, Zhejiang, Peoples R China 2.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Dept Integrated Chinese & Western Med, Canc Hosp, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Beijing, Peoples R China 4.Chinese Med Univ, Clin Coll Zhejiang 2, 548 Binwen Rd, Hangzhou, Zhejiang, Peoples R China 5.Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp, Dept Nutr,Inst Canc Res & Basic Med Sci,Canc Hosp, Hangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Yi,Zhang, Runzhe,Zhang, Xinjie,et al. Curcumin may reverse 5-fluorouracil resistance on colonic cancer cells by regulating TET1-NKD-Wnt signal pathway to inhibit the EMT progress[J]. BIOMEDICINE & PHARMACOTHERAPY,2020,129. |
| APA | Lu, Yi,Zhang, Runzhe,Zhang, Xinjie,Zhang, Bo,&Yao, Qinghua.(2020).Curcumin may reverse 5-fluorouracil resistance on colonic cancer cells by regulating TET1-NKD-Wnt signal pathway to inhibit the EMT progress.BIOMEDICINE & PHARMACOTHERAPY,129. |
| MLA | Lu, Yi,et al."Curcumin may reverse 5-fluorouracil resistance on colonic cancer cells by regulating TET1-NKD-Wnt signal pathway to inhibit the EMT progress".BIOMEDICINE & PHARMACOTHERAPY 129(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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