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Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry

文献类型：期刊论文

作者Hu, Lei1,3; Chen, Xueran1,3; Chen, Meng1,3,4; Fang, Jinman1,3; Nie, Jinfu1,2,3; Dai, Haiming1,3
刊名BIOTECHNOLOGY LETTERS
出版日期2020-09-21
关键词Circulating tumor cells Flow cytometry Fluorescence antibodies Immunomagnetic beads
ISSN号0141-5492
DOI10.1007/s10529-020-03007-8
通讯作者Nie, Jinfu(jinfunie@163.com) ; Dai, Haiming(daih@cmpt.ac.cn)
英文摘要Objective The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. Results After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be detected while less than 10% of the same cells could be detected by flow cytometry (FCM) if spiked into blood. However, in combination with CD326 and CD45 immunomagnetic beads, detection rate of MCF7 cells in blood reached 57%. For circulating tumor cells, enrichment by CD326 and CD45 immunomagnetic beads improve the detection rate from nearly undetectable to more than 24.14%. Conclusions Live CTCs in peripheral blood can be effectively and sensitively detected by using a combination of immunomagnetic beads (CD45 and CD326) and flow cytometry.
WOS关键词CANCER-PATIENTS ; BREAST-CANCER ; LUNG-CANCER ; BLOOD
资助项目100-Talent Program of Chinese Academy of Sciences ; National Natural Science Foundation of China[81572948] ; National Natural Science Foundation of China[21772201] ; National Natural Science Foundation of China[21703254] ; Anhui Medical University[LHJJ202007] ; Center of Medical Physics and Technology[LHJJ202007]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
WOS记录号WOS:000571742100001
出版者SPRINGER
资助机构100-Talent Program of Chinese Academy of Sciences ; National Natural Science Foundation of China ; Anhui Medical University ; Center of Medical Physics and Technology
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/104172]  
专题中国科学院合肥物质科学研究院
通讯作者Nie, Jinfu; Dai, Haiming
作者单位1.Chinese Acad Sci, Hefei Canc Hosp, 350 Shushanhu Rd, Hefei 230031, Anhui, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou 510530, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China
4.Univ Sci & Technol China, Hefei 230026, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Lei,Chen, Xueran,Chen, Meng,et al. Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry[J]. BIOTECHNOLOGY LETTERS,2020.
APA Hu, Lei,Chen, Xueran,Chen, Meng,Fang, Jinman,Nie, Jinfu,&Dai, Haiming.(2020).Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry.BIOTECHNOLOGY LETTERS.
MLA Hu, Lei,et al."Enrichment and detection of circulating tumor cells by immunomagnetic beads and flow cytometry".BIOTECHNOLOGY LETTERS (2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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