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Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo

文献类型：期刊论文

作者Chen, Biao1,2; Chai, Qing5; Xu, Shengmin3,4; Li, Qian1,2; Wu, Tao1; Chen, Shaopeng1; Wu, Lijun1,2,3,4
刊名ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
出版日期2020-11-01
卷号204
关键词Silver nanoparticles Lung cellular senescence COX2 SASP Human respiratory system
ISSN号0147-6513
DOI10.1016/j.ecoenv.2020.111070
通讯作者Xu, Shengmin(shmxu@ahu.edu.cn) ; Wu, Lijun(ljw@ipp.ac.cn)
英文摘要Silver nanoparticles (AgNPs) are widely used as antimicrobial agents and resulted in their accumulation in environment. The purpose of this study was to investigate the detailed molecular mechanisms underlying AgNP-induced lung cellular senescence which has been proposed as a pathogenic driver of chronic lung disease. Herein, we demonstrate that exposure to AgNPs elevates multiple senescence biomarkers in lung cells, with cell cycle arrest in the G2/M phase, and potently activates genes of the senescence-associated secretory phenotype (SASP) in human fetal lung fibroblast cell line MRCS. Fluorescence-based assay also reveals that apoptosis induced by AgNPs is associated with senescence. Furthermore, we show that AgNPs cause premature senescence through an increase in transcription factor nuclear factor kappa B (NF-kappa B), cyclooxygenase-2 (COX2) expression and overproduction of prostaglandin E2 (PGE2) in lung cells. Inhibition of COX2 reduces AgNPs-induced senescence to a normal level. Moreover, AgNPs also induce upregulation of COX2 and accelerate lung cellular senescence in vivo and cause mild fibrosis in the lung tissue of mice. Taken together, our studies support a critical role of AgNPs in the induction of lung cellular senescence via the upregulation of the COX2/PGE2 intracrine pathway, and suggest the adverse effects to the human respiratory system.
WOS关键词DNA-DAMAGE ; RAT LUNG ; EXPRESSION ; INFLAMMATION ; APOPTOSIS ; CELLS ; IDENTIFICATION ; FIBROBLASTS ; MECHANISMS ; PHENOTYPE
资助项目National Natural Science Foundation of China[31670860] ; National Natural Science Foundation of China[11835014] ; Recruitment Program for Leading Talent Team of Anhui Province[2019-16] ; National Basic Research 973 Program[2014CB932002] ; Key Program of 13th five-year plan, CASHIPS[KP-2017-05]
WOS研究方向Environmental Sciences & Ecology ; Toxicology
语种英语
WOS记录号WOS:000574855100002
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
资助机构National Natural Science Foundation of China ; Recruitment Program for Leading Talent Team of Anhui Province ; National Basic Research 973 Program ; Key Program of 13th five-year plan, CASHIPS
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/104314]  
专题中国科学院合肥物质科学研究院
通讯作者Xu, Shengmin; Wu, Lijun
作者单位1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Sch Environm Sci & Optoelect Technol, Hefei 230026, Anhui, Peoples R China
3.Anhui Univ, Inst Phys Sci & Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Anhui, Peoples R China
4.Anhui Univ, Inst Informat Technol, Informat Mat & Intelligent Sensing Lab Anhui Prov, Hefei 230601, Anhui, Peoples R China
5.Qingdao Municipal Ctr Dis Prevent & Control, Clin Lab Microorganism & Parasite, Qingdao 266033, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Biao,Chai, Qing,Xu, Shengmin,et al. Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,204.
APA Chen, Biao.,Chai, Qing.,Xu, Shengmin.,Li, Qian.,Wu, Tao.,...&Wu, Lijun.(2020).Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,204.
MLA Chen, Biao,et al."Silver nanoparticle-activated COX2/PGE2 axis involves alteration of lung cellular senescence in vitro and in vivo".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 204(2020).

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来源：合肥物质科学研究院

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