An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein
文献类型：期刊论文
| 作者 | Shen, Changming2,3; Wang, Lin4; Zhang, Hongyan2,3; Liu, Shaojuan1; Jiang, Jianwei2,3 |
| 刊名 | FRONTIERS IN CHEMISTRY
 |
| 出版日期 | 2020-09-16 |
| 卷号 | 8 |
| 关键词 | sandwich immunosensor alpha-fetoprotein electrochemical analysis cancer biomarker glassy carbon electrode |
| ISSN号 | 2296-2646 |
| DOI | 10.3389/fchem.2020.589560 |
| 通讯作者 | Jiang, Jianwei(swpru5v2@21cn.com) |
| 英文摘要 | The synthesis of Au nanocubes is used to label alpha-fetoprotein antibody (anti-AFP) and horseradish peroxidase (HRP) to form an immune complex for antibody detection. Graphene oxide-methylene blue-gold nanoparticles (GO-MB-AuNPs) nanocomposites were used as the immunosensing platform. This proposed sandwich-type immunoassay shows good performance. This method establishes a feasible amperometric immunoassay method for sensitive analysis of AFP in serum samples. Under the optimal experimental conditions, the DPV current response of the immunosensor is proportional to the logarithmic value of the AFP concentration. The linear detection range can achieve to 0.005-20 ng/mL with a detection limit of 1.5 pg/mL. The proposed immunosensor has good precision, selectivity and stability, and can be used for AFP determination in clinical tests. |
| WOS关键词 | IMMUNOASSAY ; STRATEGY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000576287200001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/104491]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Jiang, Jianwei |
| 作者单位 | 1.Third Peoples Hosp Hangzhou, Hangzhou, Peoples R China 2.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Hangzhou, Peoples R China 3.Chinese Acad Sci, Inst Canc & Basic Med IBMC, Hangzhou, Peoples R China 4.Zhejiang Chinese Med Univ, Affiliated Hosp 3, Hangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shen, Changming,Wang, Lin,Zhang, Hongyan,et al. An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein[J]. FRONTIERS IN CHEMISTRY,2020,8. |
| APA | Shen, Changming,Wang, Lin,Zhang, Hongyan,Liu, Shaojuan,&Jiang, Jianwei.(2020).An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein.FRONTIERS IN CHEMISTRY,8. |
| MLA | Shen, Changming,et al."An Electrochemical Sandwich Immunosensor Based on Signal Amplification Technique for the Determination of Alpha-Fetoprotein".FRONTIERS IN CHEMISTRY 8(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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