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Development of a serum miRNA panel for detection of early stage non-small cell lung cancer

文献类型：期刊论文

作者Ying, Lisha1,2; Du, Lingbin2,3; Zou, Ruiyang4; Shi, Lei2,5; Zhang, Nan1,2; Jin, Jiaoyue2,6; Xu, Chenyang2; Zhang, Fanrong2,7; Zhu, Chen2,3; Wu, Junzhou1,2
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2020-10-06
卷号117
ISSN号0027-8424
关键词blood biomarker microRNA early detection
DOI10.1073/pnas.2006212117
通讯作者Su, Dan(sudan@zjcc.org.cn)
英文摘要Minimally invasive testing for early detection of lung cancer to improve patient survival is a major unmet clinical need. This study aimed to develop and validate a serum multi-microRNA (multimiR) panel as a minimally invasive test for early detection of nonsmall cell lung cancer (NSCLC) regardless of smoking status, gender, and ethnicity. Our study included 744 NSCLC cases and 944 matched controls, including smokers and nonsmokers, male and female, with Asian and Caucasian subjects. Using RT-qPCR and a tightly controlled workflow, we quantified the absolute expression of 520 circulating microRNAs (miRNAs) in a Chinese cohort of 180 early stage NSCLC cases and 216 healthy controls (male smokers). Candidate biomarkers were verified in two case-control cohorts of 432 Chinese and 218 Caucasians, respectively (including females and nonsmokers). A multimiR panel for NSCLC detection was developed using a twofold cross-validation and validated in three additional Asian cohorts comprising 642 subjects. We discovered 35 candidate miRNA biomarkers, verified 22 of them, and developed a five-miR panel that detected NSCLC with area under curve (AUC) of 0.936-0.984 in the discovery and verification cohorts. The panel was validated in three independent cohorts with AUCs of 0.973, 0.916, and 0.917. The sensitivity of five-miR test was 81.3% for all stages, 82.9% for stages I and II, and 83.0% for stage I NSCLC, when the specificity is at 90.7%. We developed a minimally invasive five-miR serum test for detecting early stage NSCLC and validated its performance in multiple patient cohorts independent of smoking status, gender, and ethnicity.
WOS关键词FALSE DISCOVERY RATE ; MICRORNA ; SIGNATURE ; IDENTIFICATION ; STATISTICS ; EXPRESSION ; DIAGNOSIS ; MODEL ; TIME
资助项目Major Science and Technology Project of Zhejiang Province of China[2014C03029] ; Major Science and Technology Project of Zhejiang Province of China[2020C03023] ; Public Welfare Technology Foundation of Zhejiang Province of China[2017C34001] ; National Natural Science Foundation of China[81972917] ; Major Science and Technology Project of Medical and Health of Zhejiang Province of China[WKJ-ZJ-1902] ; Zhejiang high-level innovative talent program ; 1022 programof Zhejiang Cancer Hospital
WOS研究方向Science & Technology - Other Topics
语种英语
出版者NATL ACAD SCIENCES
WOS记录号WOS:000579045200009
资助机构Major Science and Technology Project of Zhejiang Province of China ; Public Welfare Technology Foundation of Zhejiang Province of China ; National Natural Science Foundation of China ; Major Science and Technology Project of Medical and Health of Zhejiang Province of China ; Zhejiang high-level innovative talent program ; 1022 programof Zhejiang Cancer Hospital
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/104627]  
专题中国科学院合肥物质科学研究院
通讯作者Su, Dan
作者单位1.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Canc Res Inst, Hangzhou 310022, Zhejiang, Peoples R China
2.Chinese Acad Sci, Inst Canc & Basic Med, Hangzhou 310022, Zhejiang, Peoples R China
3.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Zhejiang Canc Ctr, Hangzhou 310022, Zhejiang, Peoples R China
4.DMiRXES Hangzhou Biotechnol Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China
5.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Radiol Dept, Hangzhou 310022, Zhejiang, Peoples R China
6.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Pathol, Hangzhou 310022, Zhejiang, Peoples R China
7.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Breast Surg, Hangzhou 310022, Zhejiang, Peoples R China
8.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Chemotherapy, Hangzhou 310022, Zhejiang, Peoples R China
9.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Clin Lab, Hangzhou 310022, Zhejiang, Peoples R China
10.Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp, Dept Thorac Surg, Hangzhou 310022, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Ying, Lisha,Du, Lingbin,Zou, Ruiyang,et al. Development of a serum miRNA panel for detection of early stage non-small cell lung cancer[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117.
APA Ying, Lisha.,Du, Lingbin.,Zou, Ruiyang.,Shi, Lei.,Zhang, Nan.,...&Su, Dan.(2020).Development of a serum miRNA panel for detection of early stage non-small cell lung cancer.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117.
MLA Ying, Lisha,et al."Development of a serum miRNA panel for detection of early stage non-small cell lung cancer".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117(2020).

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来源：合肥物质科学研究院

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