Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model
文献类型：期刊论文
| 作者 | Xiao, Boduan1; Ying, Chang1; Chen, Yongyi2; Huang, Fang3; Wang, Binrong1; Fang, Huiling1; Guo, Wan1; Liu, Tao4; Zhou, Xiumei1; Huang, Biao1 |
| 刊名 | JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
 |
| 出版日期 | 2020-10-14 |
| ISSN号 | 1582-1838 |
| 关键词 | CD55-TMn CEA doxorubicin hydrochloride liver cancer oncolytic virotherapy |
| DOI | 10.1111/jcmm.15966 |
| 通讯作者 | Wang, Yigang(wangyigang43@163.com) |
| 英文摘要 | Oncolytic adenovirus (OA) has attracted increasing attention due to their specific proliferation in tumour cells and resulting in lysis of tumour cells. To further improve the antitumour effect of OA, in this study, we combined CD55-TRAIL-IETD-MnSOD (CD55-TMn), a CEA-controlled OA constructed previously, and chemotherapy to investigate their synergistic effect and possible mechanisms. MTT assay was performed to detect antitumour effects. Hoechst 33 342 and flow cytometric analysis were used to examine cell apoptosis. Western blotting was performed to examine cell pyroptosis and apoptosis mechanism. Animal experiment was used to detect antitumour effect of doxorubicin hydrochloride (Dox) combined with CD55-TMn in vivo. We firstly found that Dox promotes gene expression mediated by CEA-regulated OA and virus progeny replication by activating phosphorylation of Smad3, and Dox can enhance antitumour effect of CEA-regulated CD55-TMn by promoting cell apotopsis and cell pyroptosis. Thus, our results provide an experimental and theoretical basis on tumour therapy by combination treatment of the oncolytic virotherapy and chemotherapy and it is expected to become a novel strategy for liver cancer therapy. |
| WOS关键词 | MANGANESE SUPEROXIDE-DISMUTASE ; GENE-THERAPY ; IN-VITRO ; ADENOVIRUS ; TRAIL ; COMBINATION ; OVEREXPRESSION ; APOPTOSIS ; XENOGRAFT ; RECEPTOR |
| 资助项目 | Natural Science Foundation of Zhejiang Province[LY18C070002] ; National Nature Science Foundation of China[81803069] ; Grant for 521 talent project of ZSTU[2019A1515011678] ; Guangdong Provincial Natural Science Foundation of China[2019A1515011678] |
| WOS研究方向 | Cell Biology ; Research & Experimental Medicine |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000578244400001 |
| 资助机构 | Natural Science Foundation of Zhejiang Province ; National Nature Science Foundation of China ; Grant for 521 talent project of ZSTU ; Guangdong Provincial Natural Science Foundation of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/104666]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wang, Yigang |
| 作者单位 | 1.Zhejiang Sci Tech Univ, Xinyuan Inst Med & Biotechnol, Sch Life Sci & Med, Hangzhou 310018, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Zhejiang Canc Hosp, Canc Hosp,Inst Canc Res & Basic Med Sci, Hangzhou, Peoples R China 3.Zhejiang Prov Peoples Hosp, Dept Pathol, Hangzhou, Peoples R China 4.Guangdong Gen Hosp, Guangdong Acad Med Sci, Dept Otolaryngol, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, Boduan,Ying, Chang,Chen, Yongyi,et al. Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2020. |
| APA | Xiao, Boduan.,Ying, Chang.,Chen, Yongyi.,Huang, Fang.,Wang, Binrong.,...&Wang, Yigang.(2020).Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. |
| MLA | Xiao, Boduan,et al."Doxorubicin hydrochloride enhanced antitumour effect of CEA-regulated oncolytic virotherapy in live cancer cells and a mouse model".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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