Biochemical and NMR characterization of the interactions of Vav2-SH2 domain with lipids and the EphA2 juxtamembrane region on membrane
文献类型：期刊论文
| 作者 | Ge, Liang2,3; Wu, Bo3 ; Zhang, Youjia2,3; Wang, Jiarong3; Zhao, Hongxin3; Wang, Junfeng1,3
|
| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2020-10-01 |
| 卷号 | 477 |
| ISSN号 | 0264-6021 |
| DOI | 10.1042/BCJ20200300 |
| 通讯作者 | Wu, Bo(wubo@hmfl.ac.cn) ; Wang, Junfeng(junfeng@hmfl.ac.cn) |
| 英文摘要 | Vav2 is a ubiquitous guanine nucleotide exchange factor (GEF) for Rho family GTPases that is involved in regulating a wide range of biological processes. It interacts with several tyrosine-phosphorylated cell surface receptors, including the Eph family receptors, through its SH2 domain. The interaction of Vav2 with EphA2 is crucial for EphA2-mediated tumor angiogenesis. Here we show that Vav2-SH2 domain is a lipid-binding module that can recognize PI(4,5) P2 and PI(3,4,5)P3 lipids weakly but specifically. The specific lipid-binding site in Vav2-SH2 domain was identified by NMR chemical shift perturbation experiments using the head groups of PI(4,5)P2 and PI(3,4,5)P3, both of which bind to Vav2-SH2 with millimolar binding affinities. In addition, the interaction between Vav2-SH2 and the phosphorylated juxtamembrane region (JM) of EphA2 (Y594 phosphorylated) was investigated using NMR techniques. Furthermore, by using a nickel-lipid containing peptide-based nanodiscs system, we studied the binding of Vav2-SH2 to the phosphorylated JM region of EphA2 on lipid membrane and uncovered a role of membrane environment in modulating this protein-protein recognition. |
| WOS关键词 | PHOSPHOLIPID-BILAYER NANODISCS ; STRUCTURAL BASIS ; SH2 DOMAINS ; EXCHANGE ; BINDING ; IDENTIFICATION ; AFFINITY ; PEPTIDE ; COMPLEX ; VAV-2 |
| 资助项目 | National Natural Science Foundation of China[21673244] ; National Natural Science Foundation of China[U1532269] ; National Natural Science Foundation of China[U1632274] ; National Natural Science Foundation of China[U1732158] ; Ministry of Science and Technology of China[2016YFA0400901] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000582387100009 |
| 出版者 | PORTLAND PRESS LTD |
| 资助机构 | National Natural Science Foundation of China ; Ministry of Science and Technology of China |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/104814]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Wu, Bo; Wang, Junfeng |
| 作者单位 | 1.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Liang,Wu, Bo,Zhang, Youjia,et al. Biochemical and NMR characterization of the interactions of Vav2-SH2 domain with lipids and the EphA2 juxtamembrane region on membrane[J]. BIOCHEMICAL JOURNAL,2020,477. |
| APA | Ge, Liang,Wu, Bo,Zhang, Youjia,Wang, Jiarong,Zhao, Hongxin,&Wang, Junfeng.(2020).Biochemical and NMR characterization of the interactions of Vav2-SH2 domain with lipids and the EphA2 juxtamembrane region on membrane.BIOCHEMICAL JOURNAL,477. |
| MLA | Ge, Liang,et al."Biochemical and NMR characterization of the interactions of Vav2-SH2 domain with lipids and the EphA2 juxtamembrane region on membrane".BIOCHEMICAL JOURNAL 477(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。