A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection
文献类型：期刊论文
| 作者 | 朱灵( zhu ling)
|
| 刊名 | Micromachines
 |
| 出版日期 | 2020-02-11 |
| 卷号 | 11 |
| 关键词 | in situ PCR molecular diagnosis microfluidic chip POC device ZIKV |
| DOI | 10.3390/mi11020186 |
| 通讯作者 | Yang, Ke(keyang@rntek.cas.cn) ; Zhu, Ling(zhul@aiofm.ac.cn) |
| 英文摘要 | The outbreak of Zika virus (ZIKV) has posed a great challenge to public health in recent years. To address the urgent need of ZIKV RNA assays, we integrate the microfluidic chip embedded with chitosan-modified silicon dioxide capillaries, smartphone-based detection unit to be a C-3-system for the rapid extraction and detection of ZIKV RNA. The C-3-system is characterized by: (1) four chitosan-modified silicon dioxide capillaries integrated in the microfluidic chip for target ZIKV RNA enrichment and "in situ PCR" (polymerase chain reaction) amplification; (2) smartphone-based point of care (POC) device consisting of a pneumatic subsystem for controlling the nucleic acid extraction processes in the microfluidic chip, a heating subsystem for sample lysis and PCR amplification, and an optical subsystem for signal acquisition. The entire detection processes including sample lysis, ZIKV RNA enrichment, and reverse-transcription polymerase chain reaction (RT-PCR) is achieved in the microfluidic chip. Moreover, PCR buffers can be directly loaded into the chitosan-modified silicon dioxide capillaries for "in situ PCR", in which the captured ZIKV RNA is directly used for downstream PCR without any loss. ZIKV RNA extracted by the C-3-system can be successfully recovered at very low concentrations of 50 transducing units (TU)/mL from crude human saliva. This means that our method of detecting viremia in patients infected with ZIKV is reliable. |
| WOS关键词 | NUCLEIC-ACID EXTRACTION ; WHOLE-BLOOD ; AMPLIFICATION ; DNA ; CAPTURE ; IDENTIFICATION ; ADSORPTION ; EFFICIENT ; VIRUS |
| 资助项目 | Class C talent fund project of Chinese Academy of Sciences[CXJJ-19-C03] ; Dean's Fund of Hefei Institute of Physical Science, China[YZJJ2018QN22] ; Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui[OMST201701] ; Key Research and Development Program of Anhui[1804a09020051] ; Key Research and Development Program of Anhui[1804c04020335] ; Key Research and Development Program of Anhui[201904c03020001] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489] |
| WOS研究方向 | Science & Technology - Other Topics ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:000520181500075 |
| 出版者 | MDPI |
| 资助机构 | Class C talent fund project of Chinese Academy of Sciences ; Dean's Fund of Hefei Institute of Physical Science, China ; Open Subject of Key Laboratory of Optoelectronic Materials Science and Technology of Anhui ; Key Research and Development Program of Anhui ; Youth Innovation Promotion Association of the Chinese Academy of Sciences |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/92924]  |
| 专题 | 合肥物质科学研究院_中科院安徽光学精密机械研究所 |
| 通讯作者 | 朱灵( zhu ling) |
| 推荐引用方式 GB/T 7714 | Zhu L. A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection[J]. Micromachines,2020,11. |
| APA | 朱灵.(2020).A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection.Micromachines,11. |
| MLA | 朱灵."A Novel Microfluidic Device Integrated with Chitosan-Modified Capillaries for Rapid ZIKV Detection".Micromachines 11(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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