Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma
文献类型：期刊论文
| 作者 | 朱灵(zhu ling)
|
| 刊名 | Journal of Cancer
 |
| 出版日期 | 2020-10-17 |
| 卷号 | 11 |
| 关键词 | circulating microRNA endogenous control normalization in RT-qPCR hepatocellular carcinoma |
| ISSN号 | 1837-9664 |
| DOI | 10.7150/jca.48903 |
| 通讯作者 | Jia, Wei-Dong(jwd1968@sina.com) ; Zhu, Ling(zhul@aiofm.ac.cn) |
| 英文摘要 | Background: Circulating microRNAs (miRNAs) have proved to be promising biomarkers for early diagnosis and therapeutic monitoring in cancers. Particularly for hepatocellular carcinoma (HCC), detection of circulating miRNA biomarkers as a new diagnostic approach has been written into the latest Guidelines for Diagnosis and Treatment of Primary Liver Cancer in China (2019 edition). However, no general consensus on an ideal endogenous normalizer for circulating miRNAs quantification has been reached, so it will affect the accuracy of quantitative results. In this study, we aim to identify a stable endogenous normalizer for analyzing circulating miRNAs. Methods: Candidate miRNAs were selected by screening dataset GSE104310, as well as data statistics and analysis. Five commonly reference genes were chosen for further comparison and verification. Then, the expression levels of these genes in serum were analyzed by quantitative reverse transcription PCR (RT-qPCR) among four groups, including patients diagnosed with HCC, chronic hepatitis B (CHB), liver cirrhosis, and healthy subjects. Furthermore, the stability of target genes was evaluated using geNorm, NormFinder, comparative Delta Cq programs, and validated by database. We also explored the availability of the miRNA combination, and compared the performance difference between combination and individuals, as well as the selectivity of miRNA references in the combinations. Results: 11 candidate miRNAs were obtained, and miR-4644 stood out among these miRNAs, and proved to be much more stable than other endogenous miRNAs. Further study showed that miR-4644 exhibited higher stability and expression abundance than other commonly miRNA reference controls. Finally, we discovered the combination of miR-4644 and miR-16 revealed high performance in stability when compared to miRNA individuals. Furthermore, the combination consisted of references with closer nature could give rise to amplification effects in stability. Conclusions: Our findings demonstrated that miR-4644 is an ideal endogenous normalizer for circulating microRNA quantification in hepatocellular carcinoma. Besides, combining miR-4644 with miR-16 into a whole as a reference control would greatly improve the accuracy of quantification. |
| WOS关键词 | RELIABLE REFERENCE GENES ; CANCER STATISTICS ; PCR DATA ; MICRORNAS ; SERUM ; DIAGNOSIS ; BIOMARKERS |
| 资助项目 | Science and Technology Major Program of Anhui, China[18030801139] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-STS-ZDTP-063] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000580874400026 |
| 出版者 | IVYSPRING INT PUBL |
| 资助机构 | Science and Technology Major Program of Anhui, China ; Science and Technology Service Network Initiative of Chinese Academy of Sciences |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/92925]  |
| 专题 | 合肥物质科学研究院_中科院安徽光学精密机械研究所 |
| 通讯作者 | 朱灵(zhu ling) |
| 推荐引用方式 GB/T 7714 | Zhu L. Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma[J]. Journal of Cancer,2020,11. |
| APA | 朱灵.(2020).Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma.Journal of Cancer,11. |
| MLA | 朱灵."Identification of miR-4644 as a suitable endogenous normalizer for circulating miRNA quantification in hepatocellular carcinoma".Journal of Cancer 11(2020). |
入库方式： OAI收割
来源：合肥物质科学研究院
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