A graphene oxide-based hairpin probe coupling duplex-specific nuclease signal amplification for detection and imaging of mRNA in living cells
文献类型：期刊论文
| 作者 | Xu, Wei2,3,4 ; Zhao, Aiwu2,3,4; Zuo, Fangtao2,3,4; Hussain, Hafiz Muhammad Jafar1
|
| 刊名 | TALANTA
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 195期号:无页码:732-738 |
| ISSN号 | 0039-9140 |
| 关键词 | Duplex-specific nuclease Hairpin probe Graphene oxide Signal amplification Target mRNA Living cells imaging |
| DOI | 10.1016/j.talanta.2018.11.078 |
| 英文摘要 | In situ imaging of mRNA in living cells can help to monitor the real time mRNA expression and also useful for diagnosis and prognosis of the diseases. In this study, a new strategy was designed for simple, sensitive, and selective platform to detect the mRNA levels by combining a hairpin probe-graphene oxide (HP1/GO) and duplex-specific nuclease signal amplification (DSNSA). Initially, the DNA probe was adsorbed on the surface of GO to protect it from enzymatic digestion. Then, the target mRNA (T1) was hybridized with a partial hairpin probe which formed a duplex. Finally, under the action of DSN nuclease, the ssDNA in the DNA/RNA hybrid was selectively cleaved and produced small fragments. Then, T1 triggered the next reaction cycle, constituting a new circular exponential amplification. Here, we conclude that this assay is highly sensitive for the detection of target mRNA with the lower detection limit of 1 fM under optimal conditions. Furthermore, this strategy was successfully used for imaging of mRNA in living cells. |
| WOS关键词 | FLUORESCENCE DETECTION ; QUANTITATIVE DETECTION ; GOLD NANOPARTICLE ; LABEL-FREE ; DNA ; MICRORNA ; ACID ; STRATEGY |
| 资助项目 | National Natural Science Foundation of China[61378038] ; Direction program of Hefei Center of Physical Science Technology[2018ZYFX005] ; State Key Laboratories of Transducer Technology |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000456762000096 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; Direction program of Hefei Center of Physical Science Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology ; State Key Laboratories of Transducer Technology |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/41914]  |
| 专题 | 合肥物质科学研究院_中科院合肥智能机械研究所 |
| 通讯作者 | Zhao, Aiwu |
| 作者单位 | 1.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China 2.Chinese Acad Sci, State Key Lab Transducer Technol, Hefei 230031, Anhui, Peoples R China 3.Univ Sci & Technol China, Hefei 230027, Anhui, Peoples R China 4.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Wei,Zhao, Aiwu,Zuo, Fangtao,et al. A graphene oxide-based hairpin probe coupling duplex-specific nuclease signal amplification for detection and imaging of mRNA in living cells[J]. TALANTA,2019,195(无):732-738. |
| APA | Xu, Wei,Zhao, Aiwu,Zuo, Fangtao,&Hussain, Hafiz Muhammad Jafar.(2019).A graphene oxide-based hairpin probe coupling duplex-specific nuclease signal amplification for detection and imaging of mRNA in living cells.TALANTA,195(无),732-738. |
| MLA | Xu, Wei,et al."A graphene oxide-based hairpin probe coupling duplex-specific nuclease signal amplification for detection and imaging of mRNA in living cells".TALANTA 195.无(2019):732-738. |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。