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Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway

文献类型：期刊论文

作者Ma, Jie2,7; Yu, K. N.1,3; Zhang, Hao6; Nie, Lili2,7; Cheng, Cheng5; Cui, Shujun7; Yang, Miaomiao4,7; Chen, Guodong2,7; Han, Wei2,7
刊名JOURNAL OF PHYSICS D-APPLIED PHYSICS
出版日期2020-11-11
卷号53
关键词non-thermal plasma apoptosis protective autophagy c-JUN N-terminal kinase Sestrin2
ISSN号0022-3727
DOI10.1088/1361-6463/aba56f
通讯作者Ma, Jie()
英文摘要Although non-thermal plasma (NTP) has been proved to be an effective way to kill cancer cells, the mechanism of NTP-induced cancer cell death is still not clear. In this study, we found that NTP exposure caused reactive oxygen species generation and apoptosis in A549 and MDA-MB-231 cells. Meanwhile, NTP treatment also induced autophagy, as evidenced by the formation of acidic vesicular organelles and conversion of LC3-I to LC3-II. Suppression of autophagy by chloroquine significantly increased NTP-induced cell death, indicating that NTP-induced autophagy acted in a protective role from apoptosis. Furthermore, NTP treatment significantly increased Sestrin2 (Sesn2) expression and activated the JNK signaling pathway. Knocking down Sesn2 with special siRNA enhanced NTP-induced cell death, while pretreatment with JNK inhibitor abolished the increase of Sesn2 and LC3 formation, and promoted cell apoptosis induced by NTP. These indicated that the JNK/Sesn2 pathway was involved in autophagy and apoptosis induced by NTP. These findings provided evidence that supplement with autophagy inhibition might be a useful strategy to improve the tumor cell killing effect of NTP.
WOS关键词CELL-DEATH ; ATMOSPHERIC PLASMA ; OXIDATIVE STRESS ; CANCER ; INDUCTION ; SESTRIN2 ; SURVIVAL ; AKT
资助项目National Key R&D Program of China[2019YFC0119000] ; Natural Science Fund of Anhui Province[2008085QH352] ; Chinese National Natural Science Foundation[81573093] ; Chinese National Natural Science Foundation[U1632145] ; CASHIPS Director's Fund[YZJJ2018QN19] ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong[IRF/0024]
WOS研究方向Physics
语种英语
WOS记录号WOS:000563484600001
出版者IOP PUBLISHING LTD
资助机构National Key R&D Program of China ; Natural Science Fund of Anhui Province ; Chinese National Natural Science Foundation ; CASHIPS Director's Fund ; Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) ; Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection ; State Key Laboratory in Marine Pollution, City University of Hong Kong
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/92793]  
专题健康与医学技术研究所
通讯作者Ma, Jie
作者单位1.City Univ Hong Kong, State Key Lab Marine Pollut, Kowloon Tong, Tat Chee Ave, Hong Kong 999077, Peoples R China
2.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China
3.City Univ Hong Kong, Dept Phys, Kowloon Tong, Tat Chee Ave, Hong Kong 999077, Peoples R China
4.Anhui Med Univ, Clin Pathol Ctr, Affiliated Hosp 4, Hefei 230022, Peoples R China
5.Chinese Acad Sci, Inst Plasma Phys, Hefei Inst Phys Sci, Hefei 230031, Anhui, Peoples R China
6.Xi An Jiao Tong Univ, Ctr Plasma Biomed, State Key Lab Elect Insulat & Power Equipment, Xian 710049, Shanxi, Peoples R China
7.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Jie,Yu, K. N.,Zhang, Hao,et al. Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway[J]. JOURNAL OF PHYSICS D-APPLIED PHYSICS,2020,53.
APA Ma, Jie.,Yu, K. N..,Zhang, Hao.,Nie, Lili.,Cheng, Cheng.,...&Han, Wei.(2020).Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway.JOURNAL OF PHYSICS D-APPLIED PHYSICS,53.
MLA Ma, Jie,et al."Non-thermal plasma induces apoptosis accompanied by protective autophagy via activating JNK/Sestrin2 pathway".JOURNAL OF PHYSICS D-APPLIED PHYSICS 53(2020).

入库方式： OAI收割

来源：合肥物质科学研究院

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