Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose
文献类型：期刊论文
| 作者 | Zhu, Chang-hui1,2,4,5; Fang, Zhen3; Su, Tong-chao4; Li, Xing-kang5; Liu, Qi-ying1,2 |
| 刊名 | CELLULOSE
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 25期号:4页码:2473-2485 |
| 关键词 | Mesoporous biochar Ionothermal carbonization Immobilized cellulase Kinetics analysis Stability |
| ISSN号 | 0969-0239 |
| DOI | 10.1007/s10570-018-1704-8 |
| 通讯作者 | Fang, Zhen(zhenfang@njau.edu.cn) |
| 英文摘要 | Cellulose-based biochar was prepared via ionothermal carbonization of cellulose in [Bmim]Cl with H(2)SO(4)and subsequent pyrolysis. The biochar was analyzed by a series of characterization methods, indicating that it was a kind of mesoporous carbon suitable for the adsorption of cellulase. Kinetic analysis showed that the immobilized cellulase exhibited higher affinity to carboxymethyl cellulose than free cellulase. The immobilized cellulase, at different pH and temperatures, was more stable than free cellulase. It was used to hydrolyze pretreated cellulose in [Bmim]Cl with a total reducing sugar (TRS) yield of 99.9%. The immobilized cellulase maintained activity of 74.8% after five cycles at an immobilized cellulase/cellulose weight ratio of 30:1. When the cellulose loading was increased by a factor of 5, the TRS yield decreased by only 27.5%. |
| WOS关键词 | IONIC LIQUID ; LIGNOCELLULOSIC BIOMASS ; ENZYMATIC-HYDROLYSIS ; CARBON MATERIAL ; GLUCOSE BIOSENSOR ; BETA-GLUCOSIDASE ; NANOPARTICLES ; ENZYMES ; ACID ; STRATEGIES |
| 资助项目 | Nanjing Agricultural University[68Q-0603] ; National Science Foundation of China[51536009] ; National Science Foundation of China[51576199] |
| WOS研究方向 | Materials Science ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000428925300023 |
| 出版者 | SPRINGER |
| 资助机构 | Nanjing Agricultural University ; National Science Foundation of China |
| 源URL | [http://ir.giec.ac.cn/handle/344007/22976]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Fang, Zhen |
| 作者单位 | 1.CAS Key Lab Renewable Energy, 2 Nengyuanlu, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Nengyuanlu, Guangzhou 510640, Guangdong, Peoples R China 3.Nanjing Agr Univ, Coll Engn, Biomass Grp, 40 Dianjiangtai Rd, Nanjing 210031, Jiangsu, Peoples R China 4.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, 88 Xuefulu, Kunming 650223, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,et al. Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose[J]. CELLULOSE,2018,25(4):2473-2485. |
| APA | Zhu, Chang-hui,Fang, Zhen,Su, Tong-chao,Li, Xing-kang,&Liu, Qi-ying.(2018).Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose.CELLULOSE,25(4),2473-2485. |
| MLA | Zhu, Chang-hui,et al."Cellulase immobilized on mesoporous biochar synthesized by ionothermal carbonization of cellulose".CELLULOSE 25.4(2018):2473-2485. |
入库方式： OAI收割
来源：广州能源研究所
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