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Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer

文献类型：期刊论文

作者Yu Jia-Jun3; Liu Ping4; Zeng Zhen3; Chen Ying3; Gao Wei1,3; Li Mei1,3; Wang Chen-Guang2; Huang Zheng-Xu1,3; Zhou Zhen1,3; Li Lei1,3
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
出版日期2018-04-01
卷号46期号:4页码:463-470
关键词Matrix-assisted laser desorption ionization Linear time-of-flight mass spectrometer Development Performance characterization
ISSN号0253-3820
DOI10.1016/S1872-2040(17)61077-6
通讯作者Li Lei(lileishdx@163.com)
英文摘要Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is an important analytical technique for biological macromolecules, such as proteins, peptides and nucleic acid, especially in the field of microbial identification. On the basis of previous study, a linear MALDI-TOF MS was been designed and assembled for biological applications. The instrument comprised a vacuum system, a vacuum fast sample introduction system, an optical system, a time-of-flight mass analyzer, an ion source, a data acquisition system and an electronic control system. The ion source adopted two-stage source acceleration, delayed extraction and dynamic pulse focusing technique. The time-of-flight distance of field-free drift region was about 1 m. The optical system adopted a solid-state laser with adjustable frequency of 1-2000 Hz and spots of 20-100 mu m. The angle of incidence laser was controlled at 5 degrees. A series of experiments were carried out to further evaluate the instrument performances. It could not only analyze the samples more than 199 kDa, but also achieve isotope resolution at 1000-3000 Da and up to 900 (FWHM) at 5000-17000 Da. The minimum detectable concentration of gramicidin was 10 amol mu L-1, absolute sensitivity reached up to 2.56 amol. Independent detection of saliva samples from different targets showed that the instrument had higher producibility. We identified Escherichia coli and Shigella spp., which are two common bacteria but difficult to be differentiated by mass spectrometry, showing its potential identification for clinical microorganism. In summary, this instrument can play a role on clinical examination in the near future.
WOS关键词MALDI-TOF MS ; ESCHERICHIA-COLI ; ION FORMATION ; VITEK MS ; TIME ; IDENTIFICATION ; DESORPTION/IONIZATION ; BIOTYPER ; EXTRACTION ; RESOLUTION
资助项目Guangdong Applied Science and Technology Research and Development, China[2015B020236003] ; Fundamental Research Funds for the Central Universities of China[21616341]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000434467300002
出版者ELSEVIER SCIENCE INC
资助机构Guangdong Applied Science and Technology Research and Development, China ; Fundamental Research Funds for the Central Universities of China
源URL[http://ir.giec.ac.cn/handle/344007/23417]  
专题中国科学院广州能源研究所
通讯作者Li Lei
作者单位1.Guangdong Prov Engn Res Ctr On Line Source Apport, Guangzhou 510632, Guangdong, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
3.Jinan Univ, Inst Mass Spectrometer & Atmospher Environm, Guangzhou 510632, Guangdong, Peoples R China
4.Guangzhou HeXin Instrument Co LTD, Guangzhou 510530, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Yu Jia-Jun,Liu Ping,Zeng Zhen,et al. Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2018,46(4):463-470.
APA Yu Jia-Jun.,Liu Ping.,Zeng Zhen.,Chen Ying.,Gao Wei.,...&Li Lei.(2018).Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,46(4),463-470.
MLA Yu Jia-Jun,et al."Development and Characterization of A Linear Matrix-assisted Laser Desorption Ionization Mass Spectrometer".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 46.4(2018):463-470.

入库方式： OAI收割

来源：广州能源研究所

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