Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase
文献类型：期刊论文
| 作者 | Fu, Junying1,2; Wang, Zhiyuan1,2; Luo, Wen1,2 ; Xing, Shiyou1,2; Lv, Pengmei1,2,3; Wang, Zhongming1,2; Yuan, Zhenhong1,2,3
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2018-09-19 |
| 卷号 | 10期号:37页码:30973-30982 |
| 关键词 | Sanger's reagent immobilization lipase hydrolysis enantioselectivity |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.8609225 |
| 通讯作者 | Wang, Zhiyuan(wangzy@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn) |
| 英文摘要 | Sanger's reaction, which was originally developed for amino acid detection, was utilized for enzyme immobilization. The newly synthesized polymer support, which was called polymer NO2-4-fluorostyrene-divinylbenzene (pNFD), was embedded with a Sanger's reagent-like functional group for immobilizing enzymes covalently under mild reaction conditions. Using Burkholderia cepacia lipase (BCL) as the target enzyme, the immobilization efficiency and activity of pNFD BCL reached as high as 1.2 mg.g(-1) and 33.21 U.g(-1) (a specific activity of 27 67S U.g(-1)), respectively, realizing 90% activity recovery. It also improved the optimal reaction temperature of BCL from 40 to 65 degrees C, under which its full activity could be retained for 4 h. The new carrier also widened the pH-adaptive range of BCL as 6.5-10.0, allowing the lipase to operate normally in weak acid environment. Reusability of pNFD BCL was significantly improved as almost no activity and/or enantioselectivity loss was observed in 200 h of triglyceride hydrolysis reaction and 17 batches of (R,S)-1phenylethanol resolution reaction. |
| WOS关键词 | ENZYME IMMOBILIZATION ; ORGANIC-SOLVENTS ; POTENTIAL APPLICATIONS ; HYDROPHOBIC SUPPORTS ; CATALYTIC-PROPERTIES ; BIODIESEL PRODUCTION ; MESOPOROUS SILICA ; RESOLUTION ; STABILITY ; MEDIA |
| 资助项目 | National Natural Science Foundation of China[21576260] ; Special Funds of Applied Science and Technology Research of Guangdong Province[2015B020241002] ; Science and Technology Planning Project of Guangdong Province[2016A010104008] ; Natural Science Foundation of Guangdong Province[2015A030313720] ; Natural Science Foundation of Guangdong Province[2017A010104010] |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000445439900008 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China ; Special Funds of Applied Science and Technology Research of Guangdong Province ; Science and Technology Planning Project of Guangdong Province ; Natural Science Foundation of Guangdong Province |
| 源URL | [http://ir.giec.ac.cn/handle/344007/23885]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Wang, Zhiyuan; Lv, Pengmei |
| 作者单位 | 1.Chinese Acad Sci, Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou 510640, Guangdong, Peoples R China 2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 3.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Junying,Wang, Zhiyuan,Luo, Wen,et al. Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase[J]. ACS APPLIED MATERIALS & INTERFACES,2018,10(37):30973-30982. |
| APA | Fu, Junying.,Wang, Zhiyuan.,Luo, Wen.,Xing, Shiyou.,Lv, Pengmei.,...&Yuan, Zhenhong.(2018).Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase.ACS APPLIED MATERIALS & INTERFACES,10(37),30973-30982. |
| MLA | Fu, Junying,et al."Novel Sanger's Reagent-like Styrene Polymer for the Immobilization of Burkholderia cepacia Lipase".ACS APPLIED MATERIALS & INTERFACES 10.37(2018):30973-30982. |
入库方式： OAI收割
来源：广州能源研究所
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