Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production
文献类型：期刊论文
| 作者 | Zhang, Yu1,2,3 ; Zhang, Xiaohuan1,2,3,4; Yu, Qiang1,2,3; Yuan, Zhenhong1,2,3; Chen, Xiaoyan1,2,3,5
|
| 刊名 | JOURNAL OF BIOBASED MATERIALS AND BIOENERGY
 |
| 出版日期 | 2019-12-01 |
| 卷号 | 13期号:6页码:799-805 |
| ISSN号 | 1556-6560 |
| 关键词 | Aspergillus terreus Furfural Tolerance Mutagenesis TEM |
| DOI | 10.1166/jbmb.2019.1909 |
| 通讯作者 | Yu, Qiang(yuqiang@ms.giec.ac.cn) |
| 英文摘要 | Itaconic acid (IA) is one of the most important biobased chemicals, and is generally produced by the filamentous fungi Aspergillus terreus. In order to degrade the production cost of IA, the low-cost and renewable lignocelluloses could be used for IA production. However, inhibitors including furfural derived from indispensable lignocelluloses pretreatment, can seriously hinder the microbial growth and fermentation, and microbial IA production is also no exception. Compared to Saccharomyces cerevisiae, less is known about the effects of furfural for Aspergillus terreus. Here, a furfural tolerant strain was obtained by chemical mutagenesis with diethyl sulfate (DES), and the highest furfural tolerable concentration was up to 1.3 g/L in solid medium. The growth, glucose consumption and IA production of mutant and parental strain were compared in fermentation media containing different furfural concentration, which found 0.5 g/L furfural is the critical concentration. In addition, the morphology of organelle structure was observed with transmission electron microscope and the cellular sugar content was quantified to further support the changes in cell morphology and the impact of furfural. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; ACETIC-ACID ; ENHANCED TOLERANCE ; FERMENTATION ; XYLOSE ; CELLS |
| 资助项目 | National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[51876206] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] |
| WOS研究方向 | Chemistry ; Energy & Fuels ; Materials Science |
| 语种 | 英语 |
| 出版者 | AMER SCIENTIFIC PUBLISHERS |
| WOS记录号 | WOS:000482706800008 |
| 资助机构 | National Natural Science Foundation of China ; Municipal Science and Technology Project of Guangzhou (China) |
| 源URL | [http://ir.giec.ac.cn/handle/344007/25477]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Yu, Qiang |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.SUNY Coll Environm Sci & Forestry, Dept Paper & Bioproc Engn, Syracuse, NY 13210 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Zhang, Xiaohuan,Yu, Qiang,et al. Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production[J]. JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,2019,13(6):799-805. |
| APA | Zhang, Yu,Zhang, Xiaohuan,Yu, Qiang,Yuan, Zhenhong,&Chen, Xiaoyan.(2019).Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production.JOURNAL OF BIOBASED MATERIALS AND BIOENERGY,13(6),799-805. |
| MLA | Zhang, Yu,et al."Mutant Breeding of Furfural Tolerant Aspergillus terreus for Itaconic Acid Production".JOURNAL OF BIOBASED MATERIALS AND BIOENERGY 13.6(2019):799-805. |
入库方式： OAI收割
来源：广州能源研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。