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Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production

文献类型：期刊论文

作者Miao, Changlin1,2,3; Li, Huiwen1,2,3; Zhuang, Xinshu1,2,3; Wang, Zhongming1,2,3; Yang, Lingmei1,2,3; Lv, Pengmei1,2,3,4; Luo, Wen1,2,3
刊名RSC ADVANCES
出版日期2019-09-22
卷号9期号:51页码:29665-29675
DOI10.1039/c9ra04365a
通讯作者Yang, Lingmei(yanglm@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn)
英文摘要In this work, porous cross-linked enzyme aggregates (p-CLEAs) were synthesized by the in situ co-precipitation method using CaCO3 microparticles as templates. The preparation procedure involved the immobilization of crude lipase as CLEAs via precipitation with ammonium sulfate and entrapping these lipase molecules into the CaCO3 templates, followed by DTT (dithiothreitol)-induced assembly of lipase molecules to form lipase microparticles (lipase molecules were assembled into microparticles internally using disulfide bonds within the lipase molecules as the molecular linkers and stimulated by dithiothreitol); finally, the removal of CaCO3 templates was performed by EDTA to form pores in CLEAs. The scanning electron microscopy analysis of p-CLEAs showed a porous structure. p-CLEAs showed obvious improvement in thermal stability (after incubation at 65 degrees C, p-CLEAs lipase retained 86% relative activity, while free lipase retained only 33.67%) and pH stability (p-CLEAs relative activity was over 90% while for free lipase, the relative activity ranged from 72% to 89% from pH 6 to 9) than free lipase and could hold relatively high activity retention without activity loss at 4 degrees C for more than six months. The application of p-CLEAs in producing biodiesel showed a higher degree of conversion. The conversion of fatty acid methyl ester (FAME) was 89.7%; this value was higher by approximately 7.4% compared to that of the conventional CLEAs under the optimized conditions of a methanol-oil molar ratio of 6 : 1, with a p-CLEAs lipase dose of 20% and water content of 3% at 45 degrees C for 24 h. The FAME conversion remained greater than 70% even after reusing the p-CLEAs lipase for 8 reactions. The results demonstrated that the p-CLEAs lipase is suitable for applications in the preparation of biodiesel.
WOS关键词ENZYME AGGREGATES CLEAS ; IMMOBILIZATION ; STABILITY ; OIL ; MICROSPHERES ; PARTICLES ; PAPAIN
资助项目National Key RD Program[2017YFD0601003] ; National Nature Science Foundation of China[21506217] ; Natural Science Foundation of Guangdong Province[2016A030308004] ; Open Fund of the Key Laboratory of Renewable Energy, Chinese Academy of Sciences[Y707j91001]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000487364400022
出版者ROYAL SOC CHEMISTRY
资助机构National Key RD Program ; National Nature Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Open Fund of the Key Laboratory of Renewable Energy, Chinese Academy of Sciences
源URL[http://ir.giec.ac.cn/handle/344007/25643]  
专题中国科学院广州能源研究所
通讯作者Yang, Lingmei; Lv, Pengmei
作者单位1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China
2.Chinese Acad Sci, Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
3.Guangdong Key Lab New & Renewable Energy Res & De, Guangzhou 510640, Guangdong, Peoples R China
4.Collaborat Innovat Ctr Biomass Energy, Zhengzhou 450002, Henan, Peoples R China
推荐引用方式
GB/T 7714		 Miao, Changlin,Li, Huiwen,Zhuang, Xinshu,et al. Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production[J]. RSC ADVANCES,2019,9(51):29665-29675.
APA Miao, Changlin.,Li, Huiwen.,Zhuang, Xinshu.,Wang, Zhongming.,Yang, Lingmei.,...&Luo, Wen.(2019).Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production.RSC ADVANCES,9(51),29665-29675.
MLA Miao, Changlin,et al."Synthesis and properties of porous CLEAs lipase by the calcium carbonate template method and its application in biodiesel production".RSC ADVANCES 9.51(2019):29665-29675.

入库方式： OAI收割

来源：广州能源研究所

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