Comparative metabolomic analysis of furfural stress response in Aspergillus terreus
文献类型：期刊论文
| 作者 | Zhang, Xiaohuan1,2,3,4; Zhang, Yu1,2,3 ; Qi, Wei1,2,3; Yuan, Zhenhong1,2,3; Wang, Zhongming1,2,3
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| 刊名 | CELLULOSE
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| 出版日期 | 2019-10-01 |
| 卷号 | 26期号:15页码:8227-8236 |
| 关键词 | Aspergillus terreus Furfural tolerance Metabolomics Itaconic acid Biorefinery |
| ISSN号 | 0969-0239 |
| DOI | 10.1007/s10570-019-02402-3 |
| 通讯作者 | Zhang, Yu(zhangyu@ms.giec.ac.cn) |
| 英文摘要 | Pretreatment of lignocellulosic biomass results in the generation of a great number of inhibitors, which seriously hinder microbial growth and fermentation. Furfural, one inhibitor produced, can impede Aspergillus terreus growth and itaconic acid (IA) production. In this study, chemical mutagenesis was employed to improve the tolerance of A. terreus to furfural, with results showing significant improvement in A. terreus mutant strain growth rates, furfural degradation and reduction in IA production. Metabolomic analysis was applied to establish the underlying mechanisms of furfural tolerance in A. terreus. Differences in metabolic pathways were assessed between mutant and parental strains, when exposed to 0.5 g/L furfural, which induces significantly greater toxicity to the parental strain than the mutant strain. Results show that the higher activity of beta-alanine, glutathione and tryptophan metabolism pathways in parental strains can directly affect the cell content of NAD(+), NADP(+)/NADPH and coenzyme A, which protect cells from furfural damage. Additionally, parental strain can protect cells against furfural toxicity by reducing lysine metabolism. This study identifies four metabolic pathways which are related to furfural tolerance in A. terreus. |
| WOS关键词 | SACCHAROMYCES-CEREVISIAE ; ITACONIC ACID ; BETA-ALANINE ; LIGNOCELLULOSIC BIOMASS ; LYSINE BIOSYNTHESIS ; ACETIC-ACID ; FERMENTATION ; PRETREATMENT ; TOLERANCE ; REVEALS |
| 资助项目 | National Natural Science Foundation of China[21506215] ; National Natural Science Foundation of China[21811530623] ; Municipal Science & Technology Project of Guangzhou (China)[201804010081] |
| WOS研究方向 | Materials Science ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000489757400002 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China ; Municipal Science & Technology Project of Guangzhou (China) |
| 源URL | [http://ir.giec.ac.cn/handle/344007/25843]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Zhang, Yu |
| 作者单位 | 1.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Guangdong, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China 3.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Guangdong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaohuan,Zhang, Yu,Qi, Wei,et al. Comparative metabolomic analysis of furfural stress response in Aspergillus terreus[J]. CELLULOSE,2019,26(15):8227-8236. |
| APA | Zhang, Xiaohuan,Zhang, Yu,Qi, Wei,Yuan, Zhenhong,&Wang, Zhongming.(2019).Comparative metabolomic analysis of furfural stress response in Aspergillus terreus.CELLULOSE,26(15),8227-8236. |
| MLA | Zhang, Xiaohuan,et al."Comparative metabolomic analysis of furfural stress response in Aspergillus terreus".CELLULOSE 26.15(2019):8227-8236. |
入库方式： OAI收割
来源：广州能源研究所
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