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Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse

文献类型：期刊论文

作者Li, Hailong2,3,4,5; Chen, Xindong1,2; Xiong, Lian2,3,4,5; Zhang, Liquan1,2; Chen, Xuefang2,3,4,5; Wang, Can2,3,4,5; Huang, Chao2,3,4,5; Chen, Xinde2,3,4,5
刊名BIORESOURCE TECHNOLOGY
出版日期2020-03-01
卷号299页码:6
关键词Separation High-purity xylobiose Activated carbon adsorption Ethanol-water desorption
ISSN号0960-8524
DOI10.1016/j.biortech.2019.122625
通讯作者Chen, Xinde(cxd_cxd@hotmail.com)
英文摘要The production of high-purity xylobiose from lignocellulose is an expensive and tedious process. In this work, the production of xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse was investigated. Furthermore, a simple process for the separation of xylobiose from enzymatic hydrolysate by activated carbon absorption, water washing, and ethanol-water desorption was developed. Under the optimized separation conditions, 96.77% xylobiose was adsorbed at 16% activated carbon loadings. Moreover, xylose and acetate could not be detected after washing by 3-fold volume of water. Xylobiose with 80.16% yield was eluted by 5-fold volume of 5% (v/v) ethanol-water. The reusability of activated carbon was evaluated by 5 cycles of adsorption-desorption process, suggesting that the activated carbon exhibited good reusability. The separated xylobiose sample with high-purity (97.29%) was confirmed by HPLC, ESI-MS, and NMR. Overall, this study provided a low-cost and robust technology for the production and separation of high-purity xylobiose from lignocellulose.
WOS关键词MISCANTHUS-X-GIGANTEUS ; XYLO-OLIGOSACCHARIDES ; ANTIOXIDANT ACTIVITY ; XYLOOLIGOSACCHARIDES ; REMOVAL ; AUTOHYDROLYSIS ; PURIFICATION ; ADSORPTION ; EXPRESSION ; XYLANASE
资助项目project of Guangzhou Science and Technology[201707010241] ; National Natural Science Foundation of China[31600475] ; National Natural Science Foundation of China[51876207] ; Natural Science Foundation of Guangdong Province[2016A030310124] ; Natural Science Foundation of Guangdong Province[2017A030310052] ; Natural Science Foundation of Guangdong Province[2019A1515011843] ; foundation of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development[y809jj1001]
WOS研究方向Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种英语
WOS记录号WOS:000508117300045
出版者ELSEVIER SCI LTD
资助机构project of Guangzhou Science and Technology ; National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; foundation of Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development
源URL[http://ir.giec.ac.cn/handle/344007/26343]  
专题中国科学院广州能源研究所
通讯作者Chen, Xinde
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China
3.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
4.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
5.Chinese Acad Sci, R&D Ctr Xuyi Attapulgite Appl Technol, Guangzhou Inst Energy Convers, Xuyi 211700, Peoples R China
推荐引用方式
GB/T 7714		 Li, Hailong,Chen, Xindong,Xiong, Lian,et al. Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse[J]. BIORESOURCE TECHNOLOGY,2020,299:6.
APA Li, Hailong.,Chen, Xindong.,Xiong, Lian.,Zhang, Liquan.,Chen, Xuefang.,...&Chen, Xinde.(2020).Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse.BIORESOURCE TECHNOLOGY,299,6.
MLA Li, Hailong,et al."Production, separation, and characterization of high-purity xylobiose from enzymatic hydrolysis of alkaline oxidation pretreated sugarcane bagasse".BIORESOURCE TECHNOLOGY 299(2020):6.

入库方式： OAI收割

来源：广州能源研究所

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