Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation
文献类型：期刊论文
| 作者 | Zhang, Jun2,4; Xu, Chao2,4 ; Chen, Xiaoyan2,4; Ruan, Xi1; Zhang, Yu4; Xu, Huijuan4; Guo, Ying4; Xu, Jingliang3,4; Lv, Pengmei4; Wang, Zhongming4
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| 刊名 | ENZYME AND MICROBIAL TECHNOLOGY
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| 出版日期 | 2020-05-01 |
| 卷号 | 136页码:8 |
| 关键词 | D-Psicose D-Tagatose 3-epimerase Bacillus subtilis Fermentation Wasted apple hydrolysate |
| ISSN号 | 0141-0229 |
| DOI | 10.1016/j.enzmictec.2020.109531 |
| 通讯作者 | Xu, Jingliang(xujl@zzu.edu.cn) |
| 英文摘要 | D-Psicose, as important rare sugar and epimer of D-fructose on the C-3 position, displays unique health benefits and physiological functions in various fields. The production of D-psicose is currently obtained via enzymatic bioconversion, mostly with of D-tagatose 3-epimerase. In the present study, the isomerization of D-fructose into D-psicose was achieved by constructing engineered food-grade Bacillus subtilis through fermentation. After optimizing the fermentation conditions of the recombinant strain, the yield of D-psicose reached up to 4.56 g/L, representing an over 8-fold increase in the yield compared to unoptimized conditions, with a D-fructose conversion rate of 56.26 % when the strain was cultured at 35 degrees C for 24 h with 5 g/L D-fructose and 5mM of Mn2+. In addition, 30 g of wasted apple hydrolysate were fermented by engineered B. subtilis and gained 7.76 g/L of D-psicose at a conversion rate of 25.86 % (w/w). |
| WOS关键词 | HYDROGEN-PRODUCTION ; METAL-IONS ; STRATEGY ; GLUCOSE ; EXPRESSION ; BIOMASS ; OXYGEN |
| 资助项目 | National Key R&D Program of China[2018YFB1501701] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] ; Key Project on International Cooperation of Chinese Academy of Sciences[182344KYSB20160056] ; Guangdong Province Industrial High-tech Sector Science and Technology Project[2017A010105018] ; Guangdong Special Support Program[2017TX04Z109] ; Zhengzhou Science and Technology Innovation Leading Talent Project |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000528248400004 |
| 出版者 | ELSEVIER SCIENCE INC |
| 资助机构 | National Key R&D Program of China ; Municipal Science and Technology Project of Guangzhou (China) ; Key Project on International Cooperation of Chinese Academy of Sciences ; Guangdong Province Industrial High-tech Sector Science and Technology Project ; Guangdong Special Support Program ; Zhengzhou Science and Technology Innovation Leading Talent Project |
| 源URL | [http://ir.giec.ac.cn/handle/344007/26700]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Xu, Jingliang |
| 作者单位 | 1.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong, Peoples R China 2.Univ China Acad Sci, Beijing 100049, Peoples R China 3.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jun,Xu, Chao,Chen, Xiaoyan,et al. Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation[J]. ENZYME AND MICROBIAL TECHNOLOGY,2020,136:8. |
| APA | Zhang, Jun.,Xu, Chao.,Chen, Xiaoyan.,Ruan, Xi.,Zhang, Yu.,...&Wang, Zhongming.(2020).Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation.ENZYME AND MICROBIAL TECHNOLOGY,136,8. |
| MLA | Zhang, Jun,et al."Engineered Bacillus subtilis harbouring gene of d-tagatose 3-epimerase for the bioconversion of D-fructose into D-psicose through fermentation".ENZYME AND MICROBIAL TECHNOLOGY 136(2020):8. |
入库方式： OAI收割
来源：广州能源研究所
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