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The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure

文献类型：期刊论文

作者Chen, Xindong4,5; Xiong, Lian1,2,3,5; Li, Hailong1,2,3,5; Zhang, Liquan4,5; Yuan, Ge4,5; Chen, Xuefang1,2,3,5; Wang, Can1,2,3,5; Chen, Xinde1,2,3,5
刊名CELLULOSE
出版日期2020-05-01
卷号27期号:8页码:4417-4428
关键词Cellulose Xylan inhibition Enzymatic hydrolysis Cellulase
ISSN号0969-0239
DOI10.1007/s10570-020-03087-9
通讯作者Li, Hailong(lihl@ms.giec.ac.cn) ; Chen, Xinde(cxd_cxd@hotmail.com)
英文摘要The inhibitory effect of xylan on enzymatic hydrolysis of cellulose with different ultrastructure is not clear. In this work, cellulose II, cellulose III, and amorphous cellulose were prepared from cellulose I by NaOH, ethylenediamine, and ball-milling treatment, respectively. Their crystalline structure and enzymatic hydrolyzability were systematically characterized and compared with cellulose I. Furthermore, the inhibitory effect of xylan on cellulose with different ultrastructure was investigated in detail. Results suggested that the enzymatic hydrolyzability of different cellulose increased in sequence from cellulose I (25.4%), cellulose III (41.7%), cellulose II (56.2%) to amorphous cellulose (77.4%) at enzyme loadings of 5.56 mg protein/g substrate. The enzymatic hydrolyzability of allomorphic cellulose was strongly inhibited by xylan, while a little inhibition was observed for amorphous cellulose. It was found that cellobiose released from different cellulose was decreased after the addition of xylan. At the initial hydrolysis stage, the inhibition degree was obviously increased for all of different cellulose, and then, the inhibition degree of cellulose I and cellulose II gradually decreased as enzymatic hydrolysis proceeded, while the inhibition degree of cellulose III and amorphous cellulose had changed a little. In addition, at a longer time of hydrolysis, the inhibition degree was decreased with the increase of cellulase dosage except for cellulose III, which remained approximately unchanged. Overall, this study demonstrated that the inhibitory effect of xylan on cellulose hydrolysis was substantially affected by the ultrastructure of cellulose, and thus it provides new insights for relieving the inhibitory effect of xylan by altering the ultrastructure of cellulose.
WOS关键词AMMONIA PRETREATMENT ; ENZYMES ; LIGNOCELLULOSE ; LIGNIN ; RECALCITRANCE ; EFFICIENCY ; CONVERSION ; ALLOMORPH
WOS研究方向Materials Science ; Polymer Science
语种英语
WOS记录号WOS:000528396300018
出版者SPRINGER
源URL[http://ir.giec.ac.cn/handle/344007/26800]  
专题中国科学院广州能源研究所
通讯作者Li, Hailong; Chen, Xinde
作者单位1.Chinese Acad Sci, R&D Ctr Xuyi Attapulgite Appl Technol, Guangzhou Inst Energy Convers, Xuyi 211700, Peoples R China
2.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
3.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Energy Rd, Guangzhou 510640, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xindong,Xiong, Lian,Li, Hailong,et al. The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure[J]. CELLULOSE,2020,27(8):4417-4428.
APA Chen, Xindong.,Xiong, Lian.,Li, Hailong.,Zhang, Liquan.,Yuan, Ge.,...&Chen, Xinde.(2020).The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure.CELLULOSE,27(8),4417-4428.
MLA Chen, Xindong,et al."The inhibitory effect of xylan on enzymatic hydrolysis of cellulose is dependent on cellulose ultrastructure".CELLULOSE 27.8(2020):4417-4428.

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来源：广州能源研究所

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