Transcriptome analysis reveals metabolic regulation mechanism of microalga Chlorella pyrenoidosa in response to the mixed culture with yeast Yarrowia lipolytica
文献类型：期刊论文
| 作者 | Qin, Lei1,2,3,4 ; Wei, Dong1
|
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2020-05-21 |
| 页码 | 9 |
| 关键词 | Chlorella pyrenoidosa Yarrowia lipolytica Mixed culture Transcriptome De novo analyses |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-020-02135-y |
| 通讯作者 | Wei, Dong(fewd304@scut.edu.cn) |
| 英文摘要 | The mixed culture of microalgae and yeast has been confirmed to enhance many bioprocesses including waste degradation, remediation, and biomass generation for lipid production. Our latest study showed that besides the higher yield of biomass and lipid, the mixed culture of Chlorella pyrenoidosa and Yarrowia lipolytica can achieve the higher yields of carbohydrates, protein, and higher heating value. The strengthening mechanism of the mixed culture of microalgae and yeast mostly focused on nutritional complementarity and the culture conditions in previous works. In this work, de novo transcriptome profiling and central metabolic pathway analysis of C. pyrenoidosa cells in mono microalgae culture and mixed culture were performed. The differential transcriptome analysis revealed that most biological processes of C. pyrenoidosa, such as TCA cycle, glycolysis/gluconeogenesis pathway, pentose phosphate pathway, and nitrate assimilation, were enhanced by the mixed culture with yeast at 24 h. Meanwhile, the CO2 assimilation of C. pyrenoidosa was inhibited. Deep analysis of carbon metabolism, nitrogen metabolism, and photosynthesis well illustrates the intrinsic mechanism of physiological and biochemical differences occurring in the mono microalgae culture and the mixed culture. This study unraveled metabolic regulation molecular mechanism of C. pyrenoidosa in response to the mixed culture with Y. lipolytica, and strengthens the understanding of the synergistic effect between microalgae and yeast in the mixed culture. |
| WOS关键词 | OLEAGINOUS YEAST ; RHODOTORULA-GLUTINIS ; GENE-TRANSCRIPTION ; LIPID PRODUCTION ; BIOMASS ; COCULTIVATION ; VULGARIS ; GROWTH |
| 资助项目 | National Natural Science Foundation of China[21606230] ; Key Project of Guangdong Basic and Applied Basic Research Fund (Dongguan Joint Fund)[2019B1515120002] ; SinoPec Technology Development Program[218017-1] ; SinoPec Technology Development Program[36100002-19-FW2099-0035] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000534722700004 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China ; Key Project of Guangdong Basic and Applied Basic Research Fund (Dongguan Joint Fund) ; SinoPec Technology Development Program |
| 源URL | [http://ir.giec.ac.cn/handle/344007/27128]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Wei, Dong |
| 作者单位 | 1.South China Univ Technol, Sch Food Sci & Engn, Wushan Rd 381, Guangzhou 510641, Peoples R China 2.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 3.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangzhou 510640, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Lei,Wei, Dong. Transcriptome analysis reveals metabolic regulation mechanism of microalga Chlorella pyrenoidosa in response to the mixed culture with yeast Yarrowia lipolytica[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:9. |
| APA | Qin, Lei,&Wei, Dong.(2020).Transcriptome analysis reveals metabolic regulation mechanism of microalga Chlorella pyrenoidosa in response to the mixed culture with yeast Yarrowia lipolytica.JOURNAL OF APPLIED PHYCOLOGY,9. |
| MLA | Qin, Lei,et al."Transcriptome analysis reveals metabolic regulation mechanism of microalga Chlorella pyrenoidosa in response to the mixed culture with yeast Yarrowia lipolytica".JOURNAL OF APPLIED PHYCOLOGY (2020):9. |
入库方式： OAI收割
来源：广州能源研究所
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