Lipid accumulation and eicosapentaenoic acid distribution in response to nitrogen limitation in microalga Eustigmatos vischeri JHsu-01 (Eustigmatophyceae)
文献类型：期刊论文
| 作者 | Xu, Jin3,4; Li, Tao2 ; Li, Chu-Lin2; Zhu, Shun-Ni3; Wang, Zhong-Ming3; Zeng, Eddy Y.1,4
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| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
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| 出版日期 | 2020-06-01 |
| 卷号 | 48页码:10 |
| 关键词 | Eustigmatos Eicosapentaenoic acid Nitrogen limitation Lipid Fatty acid distribution Transcriptome |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2020.101910 |
| 通讯作者 | Zeng, Eddy Y.(eddyzeng@jnu.edu.cn) |
| 英文摘要 | Eicosapentaenoic acid (C20: 5; EPA) is one of the important omega-3 long-chain polyunsaturated fatty acids for human health, found in various microalgal species. However, the mechanisms behind the distribution of EPA in lipid molecules have not been well established. An oleaginous microalga, Eustigmatos vischeri, has been considered as a potential producer of EPA. The present study was aimed at investigating the lipid accumulation and EPA distribution in E. vischeri JHsu-01 under nitrogen-limited and nitrogen-replete conditions. The results showed that nitrogen limitation could substantially restrict the growth of the microalga and inhibit EPA synthesis, but enhance the accumulation of neutral lipids. E. vischeri JHsu-01 distributed more EPA into the membrane lipids under nitrogen-replete conditions, whereas those under nitrogen-limited conditions tended to allocate EPA into storage lipids. Besides, the expression level of several key enzymes for linoleic acid synthesis was up-regulated under nitrogen-limited conditions. The expression level of fatty acid desaturase, involving Delta 5, Delta 6 and Delta 15 desaturases decreased, which was responsible for decreasing EPA content under nitrogen-limited conditions. Eicosapentaenoic acid derived from inter-esterification of membrane lipids may participate in synthesizing triacylglycerol via catalysis of phospholipid: diacylglycerol acyltransferase, resulting in high levels of EPA for storage lipids. The present study highlighted the importance of nitrogen regulation during the cultivation of microalga to induce EPA production in E. vischeri JHsu-01. Specifically, EPA distribution within the individual lipid fractions was not solely restricted by the genetics profiles of microalgae, but also altered by available nitrogen concentrations. Capsule: Synthesis and distribution of eicosapentaenoic acid are affected by nitrogen supply. |
| WOS关键词 | FATTY-ACID ; OLEAGINOUS MICROALGA ; GROWTH ; STARVATION ; LIGHT ; EPA ; TRIACYLGLYCEROLS ; METABOLISM ; STRESS |
| 资助项目 | National Natural Science Foundation of China[31602182] ; National Natural Science Foundation of China[21878291] ; Natural Science Foundation for Research Team of Guangdong Province[2016A030312007] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000539273500005 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation for Research Team of Guangdong Province |
| 源URL | [http://ir.giec.ac.cn/handle/344007/27253]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Zeng, Eddy Y. |
| 作者单位 | 1.Jinan Univ, Res Ctr Low Carbon Econ Guangzhou Reg, Guangzhou 510632, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol LMB C, Guangdong Key Lab Marine Mat Med LMMM GD, Guangzhou 510301, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 4.Jinan Univ, Sch Environm, Guangdong Key Lab Environm Pollut & Hlth, Guangzhou 511443, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Jin,Li, Tao,Li, Chu-Lin,et al. Lipid accumulation and eicosapentaenoic acid distribution in response to nitrogen limitation in microalga Eustigmatos vischeri JHsu-01 (Eustigmatophyceae)[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2020,48:10. |
| APA | Xu, Jin,Li, Tao,Li, Chu-Lin,Zhu, Shun-Ni,Wang, Zhong-Ming,&Zeng, Eddy Y..(2020).Lipid accumulation and eicosapentaenoic acid distribution in response to nitrogen limitation in microalga Eustigmatos vischeri JHsu-01 (Eustigmatophyceae).ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,48,10. |
| MLA | Xu, Jin,et al."Lipid accumulation and eicosapentaenoic acid distribution in response to nitrogen limitation in microalga Eustigmatos vischeri JHsu-01 (Eustigmatophyceae)".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 48(2020):10. |
入库方式： OAI收割
来源：广州能源研究所
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