Molecular cloning and expression ofAmCDPKfrom mangroveAvicennia marinaunder elevated temperature
文献类型：期刊论文
| 作者 | Liu, Jin; Wang, You-Shao; Cheng, Hao3,8 |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 29期号:6页码:707 |
| 关键词 | Mangrove plants Calcium-dependent protein kinase Avicennia marina Heat stress |
| ISSN号 | 0963-9292 |
| 英文摘要 | Considered as an essential calcium sensor, the calcium-dependent protein kinase (CDPK) family plays a critical part in terrestrial plants' responses to both biotic and abiotic stresses. In the study,Avicennia marinawas proved to have better heat tolerance than other species. ACDPKgene was cloned from mangrove speciesA. marinausing RACE-PCR and designated asAmCDPK. By predicting and analyzing its properties, structures and expression patterns, we found that the amino acid sequence, containing a kinase domain and four EF-hand Ca2+-binding sites, shared high identity withHandroanthus impetiginosusandSesamum indicum. Quantitative real-time PCR data analysis suggested thatAmCDPKdemonstrated significant up-regulation under heat stress. It is likely thatAmCDPKis a versatile gene involved in various stresses, including dehydration, cold, light, defense and ABA stress responses by analyzingcis-elements. It is the first time thatCDPKs from mangroves have been cloned and our results brought evidence to the effect ofAmCDPKon heat stress, which is particularly important under the background of global warming. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18290]  |
| 专题 | 南海海洋研究所_热带海洋环境国家重点实验室(LTO) |
| 作者单位 | 1.[Liu, Jin 2.[Liu, Jin 3.Wang, You-Shao 4.Chinese Acad Sci, South China Sea Inst Oceanol, State Key Lab Trop Oceanog, Guangzhou 510301, Peoples R China 5.[Liu, Jin 6.Chinese Acad Sci, Daya Bay Marine Biol Res Stn, Shenzhen 518121, Peoples R China 7.Chinese Acad Sci, Innovat Acad South China Sea Ecol & Environm Engn, Guangzhou 510301, Peoples R China 8.Wang, You-Shao |
| 推荐引用方式 GB/T 7714 | Liu, Jin,Wang, You-Shao,Cheng, Hao. Molecular cloning and expression ofAmCDPKfrom mangroveAvicennia marinaunder elevated temperature[J]. ECOTOXICOLOGY,2020,29(6):707, 717. |
| APA | Liu, Jin,Wang, You-Shao,&Cheng, Hao.(2020).Molecular cloning and expression ofAmCDPKfrom mangroveAvicennia marinaunder elevated temperature.ECOTOXICOLOGY,29(6),707. |
| MLA | Liu, Jin,et al."Molecular cloning and expression ofAmCDPKfrom mangroveAvicennia marinaunder elevated temperature".ECOTOXICOLOGY 29.6(2020):707. |
入库方式： OAI收割
来源：南海海洋研究所
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