Grouper PKR activation inhibits red-spotted grouper nervous necrosis virus (RGNNV) replication in infected cells
文献类型：期刊论文
| 作者 | Wei, Jingguang7; Zang, Shaoqing1,2,4; Li, Chen7; Zhang, Xin7; Gao, Pin5; Qin, Qiwei3,7 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2020 |
| 卷号 | 111页码:103744 |
| 关键词 | Epinephelus coioides PKR RGNNV Cellular localization Replication |
| ISSN号 | 0145-305X |
| DOI | 10.1016/j.dci.2020.103744 |
| 英文摘要 | The double-stranded RNA-activated protein kinase (PKR) is a Type I interferon (IFN) stimulated gene that has important biological and immunological functions. In viral infections, PKR inhibits or promotes viral replication. In the present study, PKR homologues of orange-spotted grouper (Epinephelus coioides) (EcPKR) were cloned and the involvement of EcPKR during Red-spotted grouper nervous necrosis virus (RGNNV) infection was investigated. EcPKR encodes a 621-amino acid polypeptide that is closely related to the equivalent protein in Larimichthys crocea. EcPKR encoded two dsRNA binding domains and a Serine/Threonine protein kinase domain. Quantitative real-time PCR (qRT-PCR) analysis indicated that EcPKR was present in all examined tissues, with higher expression in spleen, intestine and gill. When stimulated with poly(I:C), the expression of EcPKR in the grouper spleen was increased, with highest expression 12 h post stimulation. EcPKR concentration was significantly increased in RGNNV-infected cells, with highest expression at 36 h post stimulation. EcPKR is mainly present in the cytoplasm. Overexpression of EcPKR in grouper spleen (GS) cells inhibits the transcription of the RGNNV genes. Furthermore, our results show that EcPKR overexpression significantly enhances the immune response of interferon and the activation of interferon-beta (IFN-beta), interferon stimulated response element (ISRE) and nuclear factor-kappa B (NF-kappa B). Taken together, these results are important for better understanding of the function of PKR in fish and reveal its involvement in host response to immune challenges in RGNNV. |
| 资助机构 | National Key R&D Program of China [2018YFC0311302, 2018YFD0900501]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772882, 31572643]; China Agricultural Research Service [CARS-47-G16]; Science and Technology Planning Project of Guangdong Province, China [2015TQ01N118]; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences [KF2018NO3]; National High Technology Development Program of China (863)National High Technology Research and Development Program of China [2014AA093507] ; National Key R&D Program of China [2018YFC0311302, 2018YFD0900501]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31772882, 31572643]; China Agricultural Research Service [CARS-47-G16]; Science and Technology Planning Project of Guangdong Province, China [2015TQ01N118]; Open Fund of Key Laboratory of Experimental Marine Biology, Chinese Academy of Sciences [KF2018NO3]; National High Technology Development Program of China (863)National High Technology Research and Development Program of China [2014AA093507] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18365]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Peoples R China 2.Shandong Univ, Sch Life Sci, Shandong Prov Key Lab Anim Cells & Dev Biol, Qingdao 266000, Peoples R China 3.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266000, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 5.Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Peoples R China 6.Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Hainan, Peoples R China 7.South China Agr Univ, Coll Marine Sci, Joint Lab Guangdong Prov & Hong Kong Reg Marine B, Guangzhou 510642, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Jingguang,Zang, Shaoqing,Li, Chen,et al. Grouper PKR activation inhibits red-spotted grouper nervous necrosis virus (RGNNV) replication in infected cells[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2020,111:103744. |
| APA | Wei, Jingguang,Zang, Shaoqing,Li, Chen,Zhang, Xin,Gao, Pin,&Qin, Qiwei.(2020).Grouper PKR activation inhibits red-spotted grouper nervous necrosis virus (RGNNV) replication in infected cells.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,111,103744. |
| MLA | Wei, Jingguang,et al."Grouper PKR activation inhibits red-spotted grouper nervous necrosis virus (RGNNV) replication in infected cells".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 111(2020):103744. |
入库方式： OAI收割
来源：南海海洋研究所
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