Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites
文献类型：期刊论文
| 作者 | Kang, Sisi; Yang, Mei; Hong, Zhongsi4; Zhang, Liping1; Huang, Zhaoxia; Chen, Xiaoxue; He, Suhua; Zhou, Ziliang; Zhou, Zhechong; Chen, Qiuyue |
| 刊名 | ACTA PHARMACEUTICA SINICA B
 |
| 出版日期 | 2020 |
| 卷号 | 10期号:7页码:1228 |
| 关键词 | COVID-19 Coronavirus SARS-CoV-2 Nucleocapsid protein RNA binding domain Crystal structure Antiviral targeting site |
| ISSN号 | 2211-3835 |
| DOI | 10.1016/j.apsb.2020.04.009 |
| 英文摘要 | The outbreak of coronavirus disease (COVID-19) caused by SARS-CoV-2 virus continually lead to worldwide human infections and deaths. Currently, there is no specific viral protein-targeted therapeutics. Viral nucleocapsid protein is a potential antiviral drug target, serving multiple critical functions during the viral life cycle. However, the structural information of SARS-CoV-2 nucleocapsid protein remains unclear. Herein, we have determined the 2.7 angstrom crystal structure of the N-terminal RNA binding domain of SARS-CoV-2 nucleocapsid protein. Although the overall structure is similar as other reported coronavirus nucleocapsid protein N-terminal domain, the surface electrostatic potential characteristics between them are distinct. Further comparison with mild virus type HCoV-OC43 equivalent domain demonstrates a unique potential RNA binding pocket alongside the beta-sheet core. Complemented by in vitro binding studies, our data provide several atomic resolution features of SARS-CoV-2 nucleocapsid protein N-terminal domain, guiding the design of novel antiviral agents specific targeting to SARS-CoV-2. (C) 2020 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770801, 81430041, 81620108017, 81870019]; Special Fund for Scientific and Technological Innovation Strategy of Guangdong Province of China [2018B030306029, 2017A030313145]; National Key Basic Research Program, China [SQ2018YFC090075] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770801, 81430041, 81620108017, 81870019]; Special Fund for Scientific and Technological Innovation Strategy of Guangdong Province of China [2018B030306029, 2017A030313145]; National Key Basic Research Program, China [SQ2018YFC090075] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18385]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Infect Dis, Zhuhai 519000, Peoples R China 2.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Intervent Med, Zhuhai 519000, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Inst South China Sea Ecol & Environm Engn,Key Lab, Guangzhou 510301, Peoples R China 4.Sun Yat Sen Univ, Affiliated Hosp 1, Guangdong Prov Key Lab Biomed Imaging, Mol Imaging Ctr, Zhuhai 519000, Peoples R China 5.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Expt Med, Zhuhai 519000, Peoples R China 6.Sun Yat Sen Univ, Guangdong Prov Engn Res Ctr Mol Imaging, Zhuhai 519000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Kang, Sisi,Yang, Mei,Hong, Zhongsi,et al. Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites[J]. ACTA PHARMACEUTICA SINICA B,2020,10(7):1228, 1238. |
| APA | Kang, Sisi.,Yang, Mei.,Hong, Zhongsi.,Zhang, Liping.,Huang, Zhaoxia.,...&Chen, Shoudeng.(2020).Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites.ACTA PHARMACEUTICA SINICA B,10(7),1228. |
| MLA | Kang, Sisi,et al."Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites".ACTA PHARMACEUTICA SINICA B 10.7(2020):1228. |
入库方式： OAI收割
来源：南海海洋研究所
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