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Activation and Characterization of Bohemamine Biosynthetic Gene Cluster from Streptomyces sp. CB02009

文献类型：期刊论文

作者Liu, Ling; Li, Sainan; Sun, Runze; Qin, Xiangjing2; Ju, Jianhua2; Zhang, Changsheng2; Duan, Yanwen3,4; Huang, Yong3
刊名ORGANIC LETTERS
出版日期2020
卷号22期号:12页码:4614
ISSN号1523-7060
DOI10.1021/acs.orglett.0c01224
英文摘要Bohemamines (BHMs) are bacterial alkaloids containing a pyrrolizidine core with two unusual methyl groups. Herein we report the activation of BHMs biosynthesis using a ribosome engineering approach. Characterization of the bhm gene cluster reveals that nonribosomal peptide synthetase BhmJ and Baeyer-Villiger monooxygenase BhmK are responsible for the formation of the pyrrolizidine core, which is further methylated on C-7 by methyltransferase BhmG. The 9-methyl group of BHMs is instead originated from a nonproteinogenic amino acid (2S,5S)-5-methyl-proline.
资助机构NSFCNational Natural Science Foundation of China (NSFC) [81473124, 81530092]; Chinese Ministry of Education 111 Project [B0803420, 11687139] ; NSFCNational Natural Science Foundation of China (NSFC) [81473124, 81530092]; Chinese Ministry of Education 111 Project [B0803420, 11687139]
源URL[http://ir.scsio.ac.cn/handle/344004/18391]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
作者单位1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangdong Key Lab Marine Mat Med, Guangzhou 510301, Peoples R China
2.Natl Engn Res Ctr Combinatorial Biosynth Drug Dis, Changsha 410011, Hunan, Peoples R China
3.Hunan Engn Res Ctr Combinatorial Biosynth & Nat P, Changsha 410011, Hunan, Peoples R China
4.Cent South Univ, Xiangya Int Acad Translat Med, Changsha 410013, Hunan, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Ling,Li, Sainan,Sun, Runze,et al. Activation and Characterization of Bohemamine Biosynthetic Gene Cluster from Streptomyces sp. CB02009[J]. ORGANIC LETTERS,2020,22(12):4614, 4619.
APA Liu, Ling.,Li, Sainan.,Sun, Runze.,Qin, Xiangjing.,Ju, Jianhua.,...&Huang, Yong.(2020).Activation and Characterization of Bohemamine Biosynthetic Gene Cluster from Streptomyces sp. CB02009.ORGANIC LETTERS,22(12),4614.
MLA Liu, Ling,et al."Activation and Characterization of Bohemamine Biosynthetic Gene Cluster from Streptomyces sp. CB02009".ORGANIC LETTERS 22.12(2020):4614.

入库方式： OAI收割

来源：南海海洋研究所

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