Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China
文献类型：期刊论文
| 作者 | Wu, Chao; Zhao, Zhe; Liu, Yupeng; Zhu, Xinyuan; Liu, Min; Luo, Peng2; Shi, Yan |
| 刊名 | FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
 |
| 出版日期 | 2020 |
| 卷号 | 10页码:301 |
| 关键词 | Vibrios type III secretion system (T3SS) cytotoxicity effector bacterial adhesion |
| ISSN号 | 2235-2988 |
| DOI | 10.3389/fcimb.2020.00301 |
| 英文摘要 | Vibrios, which include more than 120 valid species, are an abundant and diverse group of bacteria in marine and estuarine environments. Some of these bacteria have been recognized as pathogens of both marine animals and humans, and therefore, their virulence mechanisms have attracted increasing attention. The type III secretion system (T3SS) is an important virulence determinant in many gram-negative bacteria, in which this system directly translocates variable effectors into the host cytosol for the manipulation of the cellular responses. In this study, the distribution of the T3SS gene cluster was first examined in 110Vibriostrains of 26 different species, including 98 strains isolated from coastal areas in China. Several T3SS1 genes, but not T3SS2 genes (T3SS2 alpha and T3SS2 beta), were universally detected in all the strains of four species,Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio harveyi, andVibrio campbellii. The effector coding regions within the T3SS1 gene clusters from the T3SS1-positive strains were further analyzed, revealing that variations in the effectors ofVibrioT3SS1 were observed among the fourVibriospecies, even between different strains inV. harveyi, according to their genetic organization. Importantly, Afp17, a potential novel effector that may exert a similar function as the known effector VopS in T3SS1-induced cell death, based on cytotoxicity assay results, was found in the effector coding region of the T3SS1 in someV. harveyiandV. campbelliistrains. Finally, it was revealed that differences in T3SS1-mediated cytotoxicity were dependent not only on the variations in the effectors ofVibrioT3SS1 but also on the initial adhesion ability to host cells, which is another prerequisite condition. Altogether, our results contribute to the clarification of the diversity of T3SS1 effectors and a better understanding of the differences in cytotoxicity amongVibriospecies. |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872597]; Natural Science Foundation of Jiangsu ProvinceJiangsu Planned Projects for Postdoctoral Research FundsNatural Science Foundation of Jiangsu Province [BK20171431]; Jiangsu Agriculture Science and Technology Innovation Fund [CX [19]2033]; Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS [2019]477] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872597]; Natural Science Foundation of Jiangsu ProvinceJiangsu Planned Projects for Postdoctoral Research FundsNatural Science Foundation of Jiangsu Province [BK20171431]; Jiangsu Agriculture Science and Technology Innovation Fund [CX [19]2033]; Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS [2019]477] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18394]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Marine Bioresources Sustainable Utilizat, Key Lab Appl Marine Biol Guangdong Prov, Guangzhou, Peoples R China 2.Hohai Univ, Coll Oceanog, Dept Marine Biol, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Chao,Zhao, Zhe,Liu, Yupeng,et al. Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2020,10:301. |
| APA | Wu, Chao.,Zhao, Zhe.,Liu, Yupeng.,Zhu, Xinyuan.,Liu, Min.,...&Shi, Yan.(2020).Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China.FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,10,301. |
| MLA | Wu, Chao,et al."Type III Secretion 1 Effector Gene Diversity AmongVibrioIsolates From Coastal Areas in China".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 10(2020):301. |
入库方式： OAI收割
来源：南海海洋研究所
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