Ovarian development pattern and vitellogenesis of ridgetail white prawn, Exopalaemon carinicauda
文献类型：期刊论文
| 作者 | Wang, Lin3; Guo, Qing3; Levy, Tom4; Chen, Ting1; Wu, Xugan3,5 |
| 刊名 | CELL AND TISSUE RESEARCH
 |
| 关键词 | Ovarian development Crustacean Vitellogenesis site Closed life cycle In situ hybridization |
| ISSN号 | 0302-766X |
| DOI | 10.1007/s00441-020-03223-8 |
| 英文摘要 | The ridgetail white prawnExopalaemon carinicaudahas the potential to be used as a model organism in crustacean research because it has a transparent body, available draft genome, and short life cycle. However, their ovarian development pattern remains unclear under laboratory culture conditions. This study investigated the changes of ovarian external feature, ovarian histology, gonadosomatic index (GSI), and hepatosomatic index (HSI), as well as the expression and localization of vitellogenin in the ovary and the hepatopancreas during the first ovarian development cycle ofE. carinicaudaunder laboratory-reared condition. The results demonstrated that (1) the first ovarian development cycle ofE. carinicaudacould be divided into 5 different stages in which the ovary changes its color from white to yellow during the vitellogenesis process in parallel with increasing GSI. (2) After pubertal molt, most females reached ovarian stage II while the females reached stage V after premating molt. (3) During the ovarian development, GSI increased smoothly and HSI relatively stable during the period of stages I to IV, while GSI increased but HSI decreased significantly from stages IV to V. (4) In situ hybridization (ISH) revealed thatEcVgwas slightly expressed in the oocyte cytoplasm of previtellogenic oocytes. The positive signal was mainly detected in hepatopancreatic fibrillar cells, and a strong signal was found in the hepatopancreas at stage IV. Moreover, the expression level ofEcVg-mRNA in the hepatopancreas is stage-specific, and the hepatopancreas contributes majority of vitellin precursor protein to support the ovarian development ofE. carinicauda. |
| 资助机构 | Key R&D Program fromtheMinistry of Science and Technology of China [2018YFD0900100]; Capacity Promotion Project of Shanghai Engineering and Technology Center from the Shanghai Municipal Science and Technology Commission [19DZ2284300]; Shanghai Education Commission [A1-280118-1003]; Shanghai Municipal Human Resources and Social Security Bureau [2018100] ; Key R&D Program fromtheMinistry of Science and Technology of China [2018YFD0900100]; Capacity Promotion Project of Shanghai Engineering and Technology Center from the Shanghai Municipal Science and Technology Commission [19DZ2284300]; Shanghai Education Commission [A1-280118-1003]; Shanghai Municipal Human Resources and Social Security Bureau [2018100] |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/18397]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 作者单位 | 1.Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel 2.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China 3.Shanghai Ocean Univ, Shanghai Collaborat Innovat Ctr Aquat Anim Genet, Shanghai 201306, Peoples R China 4.Shanghai Ocean Univ, Minist Agr, Ctr Res Environm Ecol & Fish Nutr, Shanghai 201306, Peoples R China 5.Chinese Acad Sci, Key Lab Trop Marine Bioresources & Ecol, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lin,Guo, Qing,Levy, Tom,et al. Ovarian development pattern and vitellogenesis of ridgetail white prawn, Exopalaemon carinicauda[J]. CELL AND TISSUE RESEARCH. |
| APA | Wang, Lin,Guo, Qing,Levy, Tom,Chen, Ting,&Wu, Xugan. |
| MLA | Wang, Lin,et al."Ovarian development pattern and vitellogenesis of ridgetail white prawn, Exopalaemon carinicauda".CELL AND TISSUE RESEARCH |
入库方式： OAI收割
来源：南海海洋研究所
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